Published in: مجله فیزیک پزشکی ایران، دوره: 9، شماره: 2
COI code: JR_IJMP-9-2_004
Paper Language: English
How to Download This Paper
For Downloading the Fulltext of CIVILICA papers please visit the orginal Persian Section of website.
Authors Radiolabeling of Ceftriaxone with 99mTc as a Targeting Radiopharmaceutical for Staphylococcus Aureus Detection in Mouse ModelAkram Fazli - Department of Microbiology, Faculty of Sciences, Zanjan Branch, Islamic Azad University, Zanjan, Iran
Mojtaba Salouti - Biology Research Center, Zanjan Branch, Islamic Azad University, Zanjan, Iran
Gholamreza Ahmadi - Department of Nuclear Medicine, Faculty of Medicine, Zanjan University of Medical Sciences, Zanjan, Iran
Fatemeh Mirshojaei - Department of Radioisotope, Nuclear Researches Center, Atomic Energy Organization of Iran, Tehran, Iran
Abstract:Introduction Bacterial infection is one of the major causes of morbidity and mortality especially in developing countries. Nuclear medicine has an important role in helping the diagnosis of deep-seated infections by developing more specific radiopharmaceuticals. The aim of this study was to evaluate 99mTc-labeling ceftriaxone as a new radiopharmaceutical for Staphylococcus aureus infection imaging in nuclear medicine. Materials and Methods Radiolabeling of ceftriaxone was carried out by adding 370 MBq of 99mTc to 10 mg of ceftriaxone in the presence of 50 µg of SnCl2.2H2O at pH=5. The radiochemical purity and stability tests at room temperature and human blood serum were evaluated with ITLC. Intramuscular infection was induced by injection of Staphylococcus aureus into the left thigh muscle of the mice. The biodistribution of 99mTc-ceftriaxone was studied in normal and infected mice at various times post-injection. Results Radiochemical purity of the product was 94.5±5.4% with a good stability at room temperature and human serum, 80.6% and 71.2% after 24 h, respectively. The biodistribution studies showed the localization of 99mTc-ceftriaxone at the site of infection with high sensitivity without any significant accumulation in vital organs. Conclusion Due to the ease of 99mTc-ceftriaxone conjugation method, high labeling efficiency, and high uptake in the infected muscle, it may provide a promising candidate as a targeting radiopharmaceutical for imaging infectious foci due to Staphylococcus aureus in nuclear medicine.
Keywords:Ceftriaxone, Infection, Radiopharmaceutical, Staphylococcus aureus, Targeting, 99mTc-radiolabeling
COI code: JR_IJMP-9-2_004
how to cite to this paper:If you want to refer to this article in your research, you can easily use the following in the resources and references section:
Fazli, Akram; Mojtaba Salouti; Gholamreza Ahmadi & Fatemeh Mirshojaei, 2012, Radiolabeling of Ceftriaxone with 99mTc as a Targeting Radiopharmaceutical for Staphylococcus Aureus Detection in Mouse Model, Iranian Journal of Medical Physics 9 (2), https://www.civilica.com/Paper-JR_IJMP-JR_IJMP-9-2_004.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Fazli, Akram; Mojtaba Salouti; Gholamreza Ahmadi & Fatemeh Mirshojaei, 2012)
Second and more: (Fazli; Salouti; Ahmadi & Mirshojaei, 2012)
For a complete overview of how to citation please review the following CIVILICA Guide (Citation)
The University/Research Center Information:
Type: Azad University
Paper No.: 4271
in University Ranking and Scientometrics the Iranian universities and research centers are evaluated based on scientific papers.
Research Info Management
Export Citation info of this paper to research management softwares
New Related Papers
- Reaction of Tomato (Solanum Lycopersicum) Genotypes to Tomato Leaf Curl Virus (Tlcv) and Their Combining Ability For Resistance against The Virus
- Role of Discipline in the Organizational Maturity: An Analysis of Pakistani Business
- Positron Emission Particle Tracking (PEPT) of Inclusions in Metals
- Synthesis and characterization of Polyaniline– Dodecylbenzenesulfonic /organo clay nanocomposites
- Thermal Behaviors of Polyamide-Layered Silicate Nanocomposites Based on 4,4'-Azodibenzoic Acid by Solution Intercalation Technique
The Above articles are recently indexed in the related subjects
Iran Scientific Advertisment Netword
Share this paper
WHAT IS COI?
COI is a national code dedicated to all Iranian Conference and Journal Papers. the COI of each paper can be verified online.