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Expression Pattern of Telomerase Reverse Transcriptase (hTERT) Variants and Bcl-2 in Peripheral Lymphocytes of Systemic Lupus Erythematosus Patients

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Year: 2020
COI code: JR_IJP-15-3_011
Paper Language: English

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Authors Expression Pattern of Telomerase Reverse Transcriptase (hTERT) Variants and Bcl-2 in Peripheral Lymphocytes of Systemic Lupus Erythematosus Patients

  Armin Attar - Department of Cardiovascular Medicine, Division of Interventional Cardiology, Shiraz University of Medical Sciences, Shiraz, Iran
  Mohsen Khosravi Maharlooei - Students’ Research Committee, Cell and Molecular Medicine Research Group, Shiraz University of Medical Sciences, Shiraz, Iran
  Mohammad Nazarnia - Internal Medicine Department, Rheumatology Division, Shiraz University of Medical Sciences, Shiraz, Iran
  Ahmad Hosseini - Institute of Cancer Research, Shiraz University of Medical Sciences, Shiraz, Iran

Abstract:

Background & Objective: It is not clear whether activated lymphocytes of patients with systemic lupus erythematosus (SLE) are more proliferative or less apoptotic. We aimed to delineate potential differences between B and T cells of SLE patients compared to healthy controls regarding the telomerase activity and apoptosis status. Methods: In this cross-sectional case control study, Blood samples were taken from 10 SLE patients and 10 healthy controls.  B and T cells were separated using magnetic cell sorting system. Telomeric repeat amplification protocol (TRAP) assay and real-time PCR were used to determine the telomerase activity and the expression of alternatively spliced variants. Results: Four patients under treatment showed significant telomerase activity in their T cells. Four of the newly diagnosed patients showed telomerase activity in their B cells (20% of all patients and 40% of new onset patients). There was no specific pattern of human telomerase reverse transcriptase variant expression within the patients’ lymphocytes. A significantly reduced expression of Bcl-2 was detected in B cells (P=0.018) and a trend toward lower Bcl-2 expression in T cells was seen in SLE patients compared to healthy controls. Conclusion: Although not definitive, our results may suggest that B cells may have more active roles during the earlier phases of the disease attack, while T cells take over when the disease reaches its chronic stages.

Keywords:

Apoptosis, Lymphocyte, Systemic Lupus Erythematosus, Telomerase

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COI code: JR_IJP-15-3_011

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Attar, Armin; Mohsen Khosravi Maharlooei; Mohammad Nazarnia & Ahmad Hosseini, 2020, Expression Pattern of Telomerase Reverse Transcriptase (hTERT) Variants and Bcl-2 in Peripheral Lymphocytes of Systemic Lupus Erythematosus Patients, IRANIAN JOURNAL of PATHOLOGY 15 (3), https://www.civilica.com/Paper-JR_IJP-JR_IJP-15-3_011.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Attar, Armin; Mohsen Khosravi Maharlooei; Mohammad Nazarnia & Ahmad Hosseini, 2020)
Second and more: (Attar; Khosravi Maharlooei; Nazarnia & Hosseini, 2020)
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Type: Medical University
Paper No.: 2097
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