Published in: فصلنامه روشهای تصفیه محیط، دوره: 7، شماره: 3
COI code: JR_JETT-7-3_001
Paper Language: English
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Authors Analysis of Suitable Signal Peptides for Designing a Secretory Thermostable Cyanide Degrading Nitrilase: An in Silico ApproachMarzieh Asadi - Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences Shiraz, Iran
Saba Gharibi - Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences Shiraz, Iran
Seyyed Hossein Khatami - Recombinant Proteins Laboratory, Department of Biochemistry, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran
Zahra Shabaninejad - Department of Nanobiotechnology, School of Basic Sciences, Tarbiat Modares University, Tehran, Iran
Farzaneh Kargar - Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences Shiraz, Iran
Fatemeh Yousefi - Department of Biological Sciences, Faculty of genetics, Tarbiat Modares University, Tehran, Iran
Abstract:Secretory production of recombinant proteins has many benefits such as solubility and ease of purification. The aim of this study was to find suitable signal peptides for secretory production of nitriles in Bacillus subtilis. The signal peptides were chosen from Signalpeptide web server. SignalP server was used to define probability of suitable signal peptides and their secretion pathways. Physico-chemical properties and solubility were predicted by ProtParam and Protein-sol, respectively. Bioinformatics analysis identified SUBF_BACSU, GUB_BACSU, SACB_BACAM and AMY_BACAM that linked to nitriles are appropriate signal peptides. Their fusion proteins could be stable, soluble and non-antigenic proteins that might have suitable secondary and tertiary structures. The recommended signal peptides by this study are appropriate for rational designing of secretory soluble nitriles. Thus, the results of present work can be useful for future experimental production of secretory and soluble nitriles
Keywords:In silico, Nitrilase, Cyanide, Signal Peptide.
COI code: JR_JETT-7-3_001
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Asadi, Marzieh; Saba Gharibi; Seyyed Hossein Khatami; Zahra Shabaninejad; Farzaneh Kargar & Fatemeh Yousefi, 2019, Analysis of Suitable Signal Peptides for Designing a Secretory Thermostable Cyanide Degrading Nitrilase: An in Silico Approach, Journal of Environmental Treatment Techniques 7 (3), https://www.civilica.com/Paper-JR_JETT-JR_JETT-7-3_001.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Asadi, Marzieh; Saba Gharibi; Seyyed Hossein Khatami; Zahra Shabaninejad; Farzaneh Kargar & Fatemeh Yousefi, 2019)
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The University/Research Center Information:
Type: Medical University
Paper No.: 2097
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