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Detection of enterotoxin-coding genes of Staphylococcus aureus isolated from hospitalized patients using a multiplex-PCR method

Credit to Download: 1 | Page Numbers 6 | Abstract Views: 38
Year: 2019
COI code: JR_TIPS-5-4_003
Paper Language: English

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Authors Detection of enterotoxin-coding genes of Staphylococcus aureus isolated from hospitalized patients using a multiplex-PCR method

  Vahid Ghanbarinejad - Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Department of Pharmacology and Toxicology, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
  Aboozar Kazemi - Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
  Mina Zareei - Pharmaceutical Sciences Research Center, Shiraz University of Medical Sciences, Shiraz, Iran Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Shiraz University of Medical Sciences, Shiraz, Iran
  Reza Heidari - Shiraz University of Medical Sciences, Pharmaceutical Sciences Research Center

Abstract:

Staphylococcus aureus (S. aureus) is a pathogen in community-acquired or hospital infections. Hence, the identification of this pathogen in clinical samples is a health concern and demands continued surveillance and close monitoring. In the current study, S. aureus strains were isolated from various clinical specimens in the Shariati Hospital, Tehran, Iran. Samples were studied to discover S. aureus enterotoxin-coding genes A (sea), B (seb), C (sec), and D (sed). It was found that 21% enterotoxigenic S. aureus harbored sea gene, 39% were carried seb gene, 37% were positive for sec-gene, and 3% were carried sed gene. None of all S. aureus strains harbored more than one of the enterotoxigenic genes. Based on the data obtained from the current study, it could be suggested that seb and sec genes are good candidates for the identification of S. aureus in clinical specimens. Further investigations are required to discover the association between these genes and the pathogenicity of this bacteria, and finally using these data in clinical settings.

Keywords:

Staphylococcus aureus, Enterotoxin genes, Pathogenicity, Multiplex-PCR

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COI code: JR_TIPS-5-4_003

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Ghanbarinejad, Vahid; Aboozar Kazemi; Mina Zareei & Reza Heidari, 2019, Detection of enterotoxin-coding genes of Staphylococcus aureus isolated from hospitalized patients using a multiplex-PCR method, Trends in Pharmaceutical Sciences 5 (4), https://www.civilica.com/Paper-JR_TIPS-JR_TIPS-5-4_003.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Ghanbarinejad, Vahid; Aboozar Kazemi; Mina Zareei & Reza Heidari, 2019)
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Type: state university
Paper No.: 16020
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