The effect of Rosa damascena aqueous extract on hippocampal tissue changes in rats with temporal lobe epilepsy

Publish Year: 1395
نوع سند: مقاله کنفرانسی
زبان: English
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ACPLMED18_085

تاریخ نمایه سازی: 20 آبان 1397

Abstract:

Introduction: Temporal lobe epilepsy accompanies neuronal degeneration in some areas of hippocampus and mossy fiber sprouting in dentate gyrus. This study was conducted to assess the preventive effect of Rosa damascene (RD) with anti oxidant potential on structural changes in hippocampus of kainic acid -epileptic rats. Materials and Methods: In this study, 28 rats were divided into sham, RD-treated sham, epileptic, and RD -treated epileptic groups. Rat model of epilepsy was induced by unilateral intrahippocampal administration of 0.8 μg kainic acid per rat. Rats received RD (500mg/kg, i.p.) daily for 1 week before surgery. Five weeks after surgery, hippocampal sections were stained with Nissl and Timm methods. Results: Induction of epilepsy by kainic acid was followed by a significant seizure intensity & RD pretreatment attenuate seizure intensity significantly (p<0.05). In addition, density of Nissl-stained neurons in CA3 area of hippocampus was significantly lower in kainic acid group versus sham (P<0.05) and RD pretreatment significantly increased it in CA3 area versus kainic acid group. Regarding mossy fiber sprouting, epileptic rats showed a higher degree of sprouting as compared to sham group and RD pretreatment significantly lowered it (p<0.05). Conclusion: RD pretreatment could preserve neurons in CA3 area of hippocampus, and could lower mossy fiber sprouting in dentate gyrus in kainic acid -induced epileptic animals.

Authors

Reihane Hashemi

Medical Student, Shahed University, Tehran, Iran

Reza Sedaghat

Department of Pathology, Shahed University, Tehran, Iran

Mehrdad Roghani

Department of Neurophysiology, Shahed University, Tehran, Iran