Detection of IL28Brs12979860 in chronic hepatitis C using zip nucleic acid probe-based real-time PCR assay: Original Article
Publish place: The 19th Annual Congress of Medical Students of Iran
Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
AMSMED19_296
تاریخ نمایه سازی: 1 دی 1397
Abstract:
Background and Objective: Interleukin-28B (IL28B) single-nucleotide polymorphisms (SNPs) constitute important host-related factors influencing the response rate to Hepatitis C virus (HCV) standard antiviral therapy. In the last few years, several new technologies for SNP detection have been developed. This study aimed to determine IL28B rs12979860 polymorphism using Real-time PCR by ZNA-probes. Material and Methods: A total of 350 HCV-infected patients were enrolled at the Department of Hepatitis & AIDS, Pasteur Institute of Iran during the 2012–2015 periods. Detecting rs12979860 was carried out using real-time PCR with specific primers and probes of the IL28B region designed using Beacon designer software. Findings: After 40 cycles of PCR amplification, the fluorescence for each allele from each sample was presented in an easily interpretable graphic form.If there was fluorescent from the FAM dye, the sample wasgenotyped as TT. If there was fluorescent from only thereporter for the HEX dye, the sample was genotyped as CCand if there was intermediate fluorescent from both dyes, thesample was genotyped as CT. Conclusion: ZNAs as newly modified oligonucleotides are ableto enhance hybridization properties of nucleic acids.The ZNA assay can be a reliable tool which due to its low LD for SNP identification, this is one of the best method for detecting this type of polymorphism
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Authors
Roohollah Fateh
Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran
Abolfazl Fateh
Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran
Mohaddeseh farhadi
Member of Student Research Committee, Qom University of Medical Science, Qom, Iran
Seyed Davar Siadat
Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran