Molecular Identification of TEM β-lactamase Gene in Klebsiella pneumoniae Strains by Multiplex PCR in Arak, Iran

have been detected in Klebsiella species and other gram negative bacilli and it have appeared as an important resistance mechanism against and opposite to β-lactam antibiotics. TEM is one of the most important ESBL genes. This study is for identifying blaTEM genes in Klebsiella pneumoniae isolated from patients in Arak, Iran. The bacterial strains used in this study included 112 Klebsiella pneumoniae that number of 112 non-repetitive clinical isolates of K. pneumonia were gathered together from hospitals and clinical centers in Arak, Iran and were selected for the molecular study from the clinical samples including blood, urine, sputum and wound over a 6 month period. DNA was extracted from all isolates that were phenotypically strains that produce ESBLs and some non-producing strains using DNA extraction kit. To ensure quality of the extracts, amplification of 16sr-RNA gene was evaluated. Screening the presence TEM genes, the extracts were analyzed by using Multi ESBLs PCR detection kit and the Multiplex PCR reactions were completed and done. PCR amplicons were detached electrophoretically on a 1.5% (w/w) agarose gel and stained with ethidium bromide. The results of phenotypic confirmatory test showed that out of 112 isolates of K. pneumoniae, 15 were TEM-positive isolates. The results of this study was shown high prevalence of bacterial resistance against β-lactam antibiotics and it indicates a warning of the future over view of public health system about using the antibiotics. This study also present the Multiplex-PCR as a simple method for detecting +ESBLs Klebsiella pneumoniae

Keywords:

Multiplex-PCR , Keywords: Klebsiella pneumoniae , drug resistance , TEM