Biological synthesis of L-dopa from Ltyrosine by native yeast isolated from the Caspian Sea

L-DOPA (L-3, 4-dihydroxyphenylalanine) is the most effective treatment for Parkinson s disease. In the present research, isolation and characterization of L-tyrosinedegrading yeast strains and evaluating their biocatalyst ability for the biological conversion of L-tyrosine into Ldopa was investigated. L-tyrosine-degrading yeast strain Mar19 with the highest ability to converting L-tyrosine into L-dopa was phenotypically and molecularly characterized and its ITS1–5.8 S–ITS2 rDNA sequence was submitted as Debaryomyces sp. strain Mar19 (GeneBank accession no. KX268664). The effect of various parameters on bioconversion L-tyrosine into Ldopa was investigated under resting cell strategy. Based on our results, the maximal L-dopa concentration (0.56 g/l with a molar yield of 40%) was achieved after a 48 hours reaction. spectrophotometer observations and mass spectroscopy as well as Fourier transform infrared spectroscopy (FTIR) analyses revealed the presence of Ldopa in the bioconversion reaction. This is the first reporton the biological synthesis of L-dopa from L-tyrosine using the genus Debaryomyces isolated from the Caspian Sea

Keywords:

L-tyrosine , L-dopa , Biological conversion , Debaryomyces sp. Strain Mar19