Biocompatibility and bioactivity study of nanofibrous electrospun chitosan containing copper doped nanoparticle bioglass

Regeneration and repair of damaged tissue and organs in bioengineering have been named tissue engineering . The tissue engineering depends on the biologic factors, the cells, and the biocompatible three-dimensional scaffolds that mimic the extracellular matrix. Fabricating artificial skins, skin substitutes and wound healing are one of the most important areas in tissue engineering. In this study, the first, copper-doped bioglass nanoparticles were prepared using the sol-gel method. Then, the chitosan nanofibers containing the copper-doped bioglass nanoparticles were fabricated with the electrospinning method. Morphology and physicochemical properties of the nano-fibers assessed by X-ray diffraction, infrared spectroscopy, and electron microscopy techniques. Antibacterial properties of the derived composite scaffold were investigated using the Kirby-Bauer test with gramnegative bacteria (Escherichia coli) and gram-positive (Staphylococcus aureus). Biocompatibility of composite scaffold was measured using the MTT method. The results of this study shown, the prepared scaffold has an appropriate fibers diameter with biocompatible and antibacterial properties, and hence potentially may be used as a wound dressing in skin tissue engineering

Keywords:

Tissue Engineering , Chitosan nanofibers , Copper , Wound-dressing , Biocompatibility , Antibacterial activity