Binding interaction of perphenazine with calf thymus DNA: a spectroscopic and molecular docking study
Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
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شناسه ملی سند علمی:
BIOCONF20_379
تاریخ نمایه سازی: 28 اردیبهشت 1398
Abstract:
In this work, study the interaction of ct-DNA (calf thymus DNA), as a main intracellular target for various small molecules, with perphenazine (PP), an antipsychotic agent, was performed using different techniques including fluorescence spectroscopy, UV-vis absorption spectroscopy, viscometry measurement and molecular docking studies. UV–vis absorption spectroscopy results revealed that the interaction of PP with ct-DNA is a groove binding mode and the binding constant (Kb) value was calculated to be 8 × 103 M-1. Competitive fluorimetric studies with methylene blue (MB) displayed that PP possibly fits into the minor groove of ct-DNA. The results of viscometric studies indicated that no significant change occurs in the length of ct-DNA and confirm the groove binding mode of PP with ct-DNA. All experimental results were confirmed by molecular docking outputs
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Authors
Reza Yekta,
Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
Samaneh Rashtbari,
Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
Gholamreza Dehghan
Department of Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran