In Silico design of multimeric antigen as a highly immunogenic peptide vaccine against Bordetella pertussis

Raising concern about adverse effects of whole-cell vaccines against Bordetella pertussis has led to a reduction in public vaccine acceptance and a consequent increase in the incidence of the whooping cough disease. Thus, efforts were made to develop acellular subunit vaccines based on key virulence factors. One of the major advantages of subunit vaccines is that they can be also used in older children and adults. Adolescents and adults become gradually susceptible, as demonstrated by the increased incidence of atypical whooping cough cases in these age groups. Therefore, in order to in-silico design of a good recombinant chimeric vaccine against Bordetella pertussis,the amino acid sequence of the pertussis toxoid and pertactin were extracted from the NCBI and UniProtKB databases. Then the high-level immunogenic and antigenic regions of pertussis toxoid and pertactin were determined using immunoinformatic programs. By assembling these epitope-rich parts, a chimeric protein was designed that in in-silico analysis, this protein exhibited a high ability to induce the immune system against Bordetella pertussis.Epitope predictions have shown that this hypothetical structure can induce B and T cells and cause high immune responses

Keywords:

Bordetella pertussis , Peptide vaccine , Bioinformatics