Association between SGSM3 gene (rs 17001868) polymorphism and breast cancer in East Azarbaijan population

Breast cancer is one of the most common types of cancer that causes many deaths among women and men every year. Despite the many advances that have been made in early diagnosis and proper treatment of this disease, the most common causes of death are breast cancer among women. According to Iran s statistics, in our country every 10 to 15 women have a chance of having breast cancer.The aim of this study was to investigate the association of SGSM3L-dependent rs17001868 polymorphism with breast cancer in the East Azarbaijan population. In this study, 100 blood samples of patients with breast cancer and 100 blood samples from healthy persons as control group were selected. Then DNA was extracted from all samples using kit of DNA extraction according to the protocol. Then, in order to ensure the quality of the DNA extracted, electrophoresis was performed and quantitated with spectrophotometer. In the next step, the specimens were amplified by PCR and electrophoresis was performed again. Ultimately, PCR products were treated with the restriction enzyme Ssp 1 (Sphaerotilus species) and electrophoresed on the agarose gel and the polymorphism was observed as bands. Data were analyzed by SPSS software Version 21 and evaluated by descriptive and chisquare test. The level of significance was less than 0.05.The results of this study indicate that the percentage of allele A is higher in healthy people, but the difference between healthy and patient is not significant. There is not probably a correlation between the increase in the A allele and the incidence of breast cancer. Therefore, the relationship between rs17001868 polymorphism and breast cancer can be revealed through more detailed studies. Given that current treatments for treating various types of cancers have serious complications, the discovery of new methods for early detection of the disease by identifying the specific biomarkers is essential and could open new therapeutic approaches.

Keywords:

Breast Cancer , Polymorphism , rs17001868 , SGSM3