Association between ADSL gene (rs 3788577)polymorphism and breast cancer in East Azarbaijan population

Breast cancer is one of the most common types of cancer that causes many deaths among women and men every year. The aim of this study was to investigate the association of ADSL-dependent rs3788577 polymorphism with breast cancer in the East Azarbaijan population.In this study, 100 blood samples of patients with breast cancer and 100 blood samples from healthy persons as control group were selected. Then DNA was extracted from all samples using kit of DNA extraction according to the protocol. In the next step, the specimens were amplified by PCR and electrophoresis was performed again. Ultimately, PCR products were treated with the restriction enzyme Hpa II * (BsiS I) (Msp I) and electrophoresed on the agarose gel and the polymorphism was observed as bands. Data were analyzed by SPSS software Version 21 and evaluated by descriptive and chi-square test. The percentage of allele A in healthy persons and patients was 85.5% and 41.5% respectively, and G allele in healthy persons and patients was 14.5% and 58.5%, respectively. The results of this study indicate that the G allele in patients is elevated by 44% in comparison to healthy persons. There is probably a correlation between the increase in the G allele (44%) and the incidence of breast cancer. Therefore, the relationship between rs3788577 polymorphism and breast cancer can be revealed through more detailed studies.

Keywords:

Breast Cancer , Polymorphism , rs3788577 , ADSL