Study of the antibiotic resistance pattern and frequency of streptomycin, trimethoprim,gentamycin, sulfonamides and chloramphenicol resistance genes in Escherichia coli isolated from urinary tract infections of women in Tabriz city

Urinary tract infection is one of the most common diseases in human societies. Unfortunately, exorbitance consumption of antibiotics has caused gradual resistance in bacteria. The aim of this research was tostudy of the antibiotic resistance pattern and frequency of streptomycin, trimethoprim, gentamycin, sulfonamides and chloramphenicol resistance genes in Escherichia coli isolated from urinary tract infections of women in Tabriz city. In this cross-sectional descriptive study, 20 samples of E. coli isolated from urinary tract infections in women were tested for surveying of frequency of aadA1, dfrA1,aac, sul1, cmlA and cat1 genes by PCR method. Furthermore, antibiogram test was performed for studying of antibiotic resistance of these isolates by Kirby Bauer method. The frequency of sul1, cat, dfrA1, aac, cmlA and aadA1 genes were 10%, 0%, 0%, 0%, 0% and 0%, respectively. The most antibiotic sensitivity was related to cotrimoxazole (60%) and nalidixic acid (45%). The most antibiotic resistance was reported to gentamicin (85%) and streptomycin (75%), respectively. The increase of antibiotic resistance can represent the exorbitance consumption of antibiotics, therefore more appropriate actions are taken for using of common therapeutic methods and doing the exact antibiogram test is an inevitable necessity before prescribing an antibiotic.

Keywords:

Escherichia coli , Antibiotic resistance , Urinary tract infection , PCR