Study of TNFR1 and TNFR2 single nucleotide polymorphisms among Iranian normal population

Tumor necrosis factor-alpha (TNF-α) is a multifunctional cytokine. TNF-α signaling compromises of two TNFR1 and TNFR2 trans-membrane receptors. polymorphisms occurs in the TNF-α or/and its receptors consequently affect the circulating pathway of TNF-α in theblood cells; resulting in scale-up susceptibility to various human diseases. This study aimed to determine the possible polymorphisms of TNFR1 and TNFR2 among the Iranian normal population with no history of diseases and susceptibility to diseases. TNFR1 and TNFR2 single nucleotide polymorphisms (SNPs) at positions 36 and 587 respectively were studied using polymerase chain reaction (PCR) and PCR-restriction fragmentlength polymorphisms (PCR-RFLR). This study involved 290 blood samples isolated from healthy individual with no history of any types of diseases including TB and other underlying diseases, Malignancy, diabetes and autoimmune diseases. Among them, 126 samples (43.44%)were isolated from males and the remind 164 (56.55%) were isolated from females. PCR-RFLR of TNFR1 detected total of 580 alleles including 441 (76.03%) allele A and 139 (23.96%) allele G. Out of 580 alleles identified in TNFR2, 208 (35.86%) alleles were T and 372 (64.13%) alleles were G. Allele comparison was showed the higher frequency of allele G in males (166; 28.62%) and females (166; 28.62%). Both T and G alleles were more frequent in females (41.89% and 14.65% respectively). In this study, A to G allele substitutions in TNFR1 (441/139) in both males and females much less occurred compare to T to G allele substitutions in TNFR2 (208/372), resulted in statistically significant TNFR2 SNPs amongIranian population.

Keywords:

TNFR1 , TNFR2 , single nucleotide polymorphisms , SNPs , Iranian population