Overview of the Survey of Aflatoxin M1 in cheese by ELISA method

Aflatoxin M1 (AFM1) is a major metabolite of aflatoxin B1 (AFB1), which is formed when animals ingest feed contaminated with aflatoxin B1. The AFB1, once ingested by the animal, is rapidly absorbed by the gastrointestinal tract and is transformed into the metabolite AFM1, which appears in the blood after 15 minutes and is then secreted in the milk by theMammary glands. The amount of AFM1 which is found in milk depends on several factors, such as animal breed, lactation period, mammary infections etc… The enzyme-linked immunosorbent assay is most widely used test to detect aflatoxins, due to its simplicity, sensitivity and adaptability. There are two types of enzyme-linked immunosorbent assay, which are direct competitive enzyme-linked immunosorbent assay and indirect competitive enzyme-linked immunosorbent assay. The following formula is used to measure the % absorbance. (Absorbance of standard or sample / absorbance of zero standards) × 100 = % absorbance the zero standard is made equal to 100 % and absorbance values are taken in percentages.

Keywords:

Aflatoxin m1 , cheese , ELISA