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Spectrometric Determination of azathioprine After Molecularly Imprinted Solid Phase Extraction based on multivariate optimization

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Authors Spectrometric Determination of azathioprine After Molecularly Imprinted Solid Phase Extraction based on multivariate optimization

  Ali Reza Taheri - Islamic Azad University – Ilam Branch, Ilam, Iran
  Nasrin Rahmatian - Islamic Azad University – Ilam Branch, Ilam, Iran

Abstract:

The selective molecularly imprinted polymer was produced using Azathioprine and methacrylic acid as a template molecule and monomer, respectively. In order to investigate the effect of independent variables on the extraction efficiency, the response surface methodology (RSM) based on Box-Behnken design (BBD) was employed. The analytical parameters such as precision, accuracy and linear working range were also determined in optimal experimental conditions and the proposed method was applied to analysis of Azathioprine. The linear dynamic range and limits of detection were 2.5-0.01 and 0.008 mg L-1 respectively. The recoveries for analyte were higher than 95% and relative standard deviation values were found to be in the range of 0.83-4.15%. This method was successfully applied for the determination of Azathioprine in biological and pharmaceutical samples

Keywords:

Azathioprine, Nano molecularly imprinted polymer, Solid-phase extraction, Spectrophotometry, Box–Behnken design

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Taheri, Ali Reza & Nasrin Rahmatian, 2016, Spectrometric Determination of azathioprine After Molecularly Imprinted Solid Phase Extraction based on multivariate optimization, The 6th National Conference and the First International Conference on Chemistry Applications in New Technologies, اصفهان, موسسه آموزش عالي جامي, https://www.civilica.com/Paper-CAAT06-CAAT06_016.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
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Type: Azad University
Paper No.: 2528
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