CIVILICA We Respect the Science
Publisher of Iranian Journals and Conference Proceedings
Paper
title

Comparison Study of the methylation moieties of the CYP1A1 gene promoter in gastric cancer patients

Credit to Download: 0 | Page Numbers 1 | Abstract Views: 122
Year: 2018
COI code: CANCERMED04_005
Paper Language: English

How to Download This Paper

For Downloading the Fulltext of CIVILICA papers please visit the orginal Persian Section of website.

Authors Comparison Study of the methylation moieties of the CYP1A1 gene promoter in gastric cancer patients

  L Sadeghi-Amiri - Department of Basic Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.
  N Nikbakhsh-Zati - Department of Surgery, Faculty of Medicine, Babol University of Medical Sciences, Babol, Iran.
  A Barzegar - Department of Basic Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.
  P Mehraban - Department of Basic Sciences, Sari Agricultural Sciences and Natural Resources University, Sari, Iran.

Abstract:

Introduction and aim: Gastric cancer is the fourth common malignancies and the second leading cause of cancer related death worldwide. DNA methylation of some genes has been considered as one of the most important changes which control organization and function of a cell. Recent studies have revealed discrete methylation pattern of P450 CYP1A1 genes which is xenobiotics detoxification enzyme in tumeric versus its neighboring normal tissue. Method: Tissues were dissected from patients who were undergone surgery. Genomic DNA extracted using phenol-chloroform method was subjected to bisulfate treatment by Qiagene kit. Desired region located in CYP1A1 gene promoter were PCR-amplified and were sequenced in both directions in Bioneer company. Statistical analysis was conducted by mann-whitney method. Results: Results showed that the methylation rates of some CpG dinucleotides are different in normal versus turmeric tissues. Statistical analysis revealed that there are significant differences between methylation rates of turmeric and normal tissues ((P<0.05%). Conclusion: Increasing the methylation rates of CpG dinucleotides present in CYP1A1 gene promoter is significantly associated with stomach cancer incidence in. Regarding to the importance of respective region in CYP1A1 gene promoter as xenobiotic and transcription factors response elements, different methylation rate in turmeric versus normal tissues would change transcription rates which might lead to cancer incidence. Screening methylation pattern of this region may be useful as tumor marker to evaluate susceptibility to stomach cancer.

Keywords:

CYP1A1, DNA methylation, Gastric cancer, Northern Iran.

Perma Link

https://www.civilica.com/Paper-CANCERMED04-CANCERMED04_005.html
COI code: CANCERMED04_005

how to cite to this paper:

If you want to refer to this article in your research, you can easily use the following in the resources and references section:
Sadeghi-Amiri, L; N Nikbakhsh-Zati; A Barzegar & P Mehraban, 2018, Comparison Study of the methylation moieties of the CYP1A1 gene promoter in gastric cancer patients, 4th International Congress of Gastrointestinal Cancer, تهران, مركز تحقيقات سرطان بيمارستان شهداي تجريش, https://www.civilica.com/Paper-CANCERMED04-CANCERMED04_005.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Sadeghi-Amiri, L; N Nikbakhsh-Zati; A Barzegar & P Mehraban, 2018)
Second and more: (Sadeghi-Amiri; Nikbakhsh-Zati; Barzegar & Mehraban, 2018)
For a complete overview of how to citation please review the following CIVILICA Guide (Citation)

Scientometrics

The University/Research Center Information:
Type: state university
Paper No.: 5712
in University Ranking and Scientometrics the Iranian universities and research centers are evaluated based on scientific papers.

Research Info Management

Export Citation info of this paper to research management softwares

New Related Papers

Iran Scientific Advertisment Netword

Share this paper

WHAT IS COI?

COI is a national code dedicated to all Iranian Conference and Journal Papers. the COI of each paper can be verified online.