Novel Binding Models for Interaction of Small Molecules to Proteins

In characterization, various aspects of macromolecular phenomena in living systems,reversible binding of small molecules to biological macromolecules such as proteins andDNA is of fundamental importance. This is illustrated by the binding curve that measuredexperimentally and analyzed by the binding models in order to characterize its physicalnature and biological importance. This article represents the results of two decades study onexperimental, theoretical, and computational aspects of this phenomenon in our group.Accordingly, the novel analysing models are introduced and the contribution of these workson improvement of our understanding of the functional chemistry of proteins is discussed.The concepts such as binding capacity, mean intrinsic binding quantities and bindingstatistical factors were presented and the experimental analyzing methods were assigned fortheir estimation from raw experimental data.

Keywords:

Ligand binding , Binding model , Mean intrinsic function , Binding capacity