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Inhibitory Effect of Graphene Oxides on Hen Egg White Lysozyme Amyloid Fibril Formation

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Year: 2018
COI code: CBC15_035
Paper Language: English

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Authors Inhibitory Effect of Graphene Oxides on Hen Egg White Lysozyme Amyloid Fibril Formation

  Mohsen Mahdavimehr - Department of biological sciences, institute for advanced studies in basic sciences (IASBS), Zanjan 45137-66731, Iran
  Ali Akbar Meratan - Department of biological sciences, institute for advanced studies in basic sciences (IASBS), Zanjan 45137-66731, Iran
  Atiyeh Ghasemi - Institute of biochemistry and biophysics, University of Tehran, Tehran, Iran
  Ali Akbar Saboury - Institute of biochemistry and biophysics, University of Tehran, Tehran, Iran

Abstract:

Protein misfolding and aggregation is related to a large number of neurodegenerative disorders including Alzheimer‟s, Parkinson‟s, and Huntington‟s diseases. Here, we exploited Graphene Oxides (GO) as an efficient inhibitor of hen egg white lysozyme (HEWL) amyloid fibrillation. A range of amyloid-specific techniques, including thioflavin T (ThT) and 1-anilino-naphthalene 8-sulfonate (ANS) fluorescence measurements and Congo red binding assay were employed to investigate the efficacy of GO for inhibition of HEWL amyloid fibrillation. Additionally, the morphology of resultant aggregates in the absence and presence of GO were analyzed by Atomic Force Microscopy (AFM). Kinetics of HEWL amyloid formation in the absence and presence of GO demonstrated a concentration-dependent decrease in ThT fluorescence. Similarly, a pronounced dose-dependent decrease in ANS fluorescence intensity and Congo red absorbance were also observed. These results were more confirmed by AFM images, suggesting that in the presence of GO formation of mature fibrillar structures was strongly inhibited. Based on obtained results, it is concluded that GO are able to act as an effective inhibitor of HEWL amyloid fibrillation.

Keywords:

Hen egg white lysozyme, Graphene Oxides, Thioflavin T, Atomic force microscopy

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COI code: CBC15_035

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Mahdavimehr, Mohsen; Ali Akbar Meratan; Atiyeh Ghasemi & Ali Akbar Saboury, 2018, Inhibitory Effect of Graphene Oxides on Hen Egg White Lysozyme Amyloid Fibril Formation, 14th Conference on Biophysical Chemistry, گرگان, دانشگاه آزاد اسلامي واحد گرگان -انجمن بيوشيمي فيزيك ايران, https://www.civilica.com/Paper-CBC15-CBC15_035.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Mahdavimehr, Mohsen; Ali Akbar Meratan; Atiyeh Ghasemi & Ali Akbar Saboury, 2018)
Second and more: (Mahdavimehr; Meratan; Ghasemi & Saboury, 2018)
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