Study of the simultaneous effect of silver nanoparticles and 3-β-hydroxybutyrate on DNA glycation process

The unique properties and performance of nanoparticles come from a variety of characteristics, including the same size as nanoparticles and biological macromolecules. Glycation of nucleotides leads to DNA damage and genetic disorders .In recent years, the antibiotic and pharmaceutical roles of silver nanoparticles have been identified in several studies . In this study, the diabetic DNA of calf thymus was treated during 28 days in a like-physiological condition along with 3-β-hydroxybutyrate keton bodies (3BHB) in presence and absence of silver nanoparticles. Then structural changes of DNA were investigated using different spectroscopic methods. The CD results show that the structural changes of glycated DNA in the presence of 3-b-hydroxybutyrate along with silver nanoparticles is less than that of Glycated DNA in the presence of only 3-beta-hydroxybutyrate. Also, the results of fluorescence spectroscopy at λex = 290 nm showed a decrease in emission fluorescence intencity (λmax 450 nm) in presence 3BHB along with silver nanoparticles. Therefore, silver nanoparticles with decrease AGEs formations can decrease the structure changes of DNA and can prevent from cleavage of DNA in diabetic conditions.

Keywords:

DNA , Silver nanoparticles , 3-B-hydroxybutyrate , Glycation