Combinatorial anticancer effects of cisplatin and herbal exteracts loaded PEG/PLA Diblock-Co-Polymer Nano-composites towards human ovarian cancer treatment in vitro

The use of biodegradable and biocompatible nanoparticles has been increasing for drug delivery applications. One of the reasons for this is the ability to accurately control the drugs release rate, long-term and steady blood retention, controlled drug-release from such systems, increased therapeutic effectiveness, reduce side effects with accurate transfusion, and etc. Among polymers, PEG/PLA diblock co-polymers have been especially noted for their biocompatibility and biodegradability, as well as the ability to form different types of nanocomposites.In this study, PEG/PLA nanocomposites were synthesized by chemical precipitation and used as cisplatin (CP) and herbal extract carriers for their controlled release against human ovarian cancer cells. Extraction of watermelon seeds (WS) and Persian olives (PO) was done by Soxhlet method and the presence of extract s phytochemicals was studied using colorimetric method. CP, WS and PO loading onto PEG/PLA nanocomposites was was done by oil-in-water emulsion method. The obtained copolymers were evaluated by FTIR, UV-Vis absorption by Nanodrop, electron microscopy. The drug loading efficiency and the drug release profiles (dialysis) were measured in vitro by a Nanodropat 215-800 nm, which showed a long-term release mechanism. MTT analysis results have shown that the as prepared nanocomposite system showed significant effect on apoptotic induction in OVCAR-3 cells. Our colorimetric results have shown that our WS and PO extracts contained phenols, flavonoids, triterpenoids, and vitamins C, etc., which caused considerable amount of cytotoxicity in cancer cells. TheCP release from PEG/PLA showed a dose- and concentration-dependent manner

Keywords:

Nanocomposite , Pharmaceutical carriers , PEG/PLA , Cisplatin , Herbal extracts , ovarian cancer