Electrochemical detection of anti breast cancer drug in bulk drug and its pharmaceutical dosage form using differential-pulse adsorptive anodic stripping

The electro-oxidative behaviour of tamoxifen (Tam) as an anti cancer drug was investigated in Britton-Robinson (BR) buffer by differential-pulse adsorptive anodic stripping (DPAdAS) technique. The anodic oxidation peak of Tam was attributed to the cyclization reaction to form the corresponding phenanthrene derivative. Oxidative stripping analysis was successfully applied to the determination of Tam in a bulk pharmaceutical formulation. In this work, to find the best conditions for taking a sharpanalytical peak concerning the electro-oxidation of Tam, the effects of different parameters such as; deposition potential, deposition time, pH and the electrocleaning conditions have been studied and optimized. Then the calibration curve was plotted in the range of 1 to 10 μM and the limits of detection (LOD) and quantitation (LOQ) were calculated to be 0.621 and 2.07 μM, respectively. The mean, standard error and relative standard deviation (RSD) for five replicates of 4.0 μM were found to be 4.1 μM, 2.65 % and 3.62 %, respectively. To estimate the application potential of the proposed method, the extraction of Tam from tablets containing 20 mg Tam were investigated and optimized. Finally, the proposed method was successfully employed for determination of Tam in spiked physiological samples.

Keywords:

Tamoxifen , DPAASV , Striping voltammetry , deposition