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H(2)O(2) induced apoptosis in human oligodendrocyte progenitor cells (OPC(s)) culture

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Year: 2012
COI code: CIGS12_0417
Paper Language: English

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Authors H(2)O(2) induced apoptosis in human oligodendrocyte progenitor cells (OPC(s)) culture

  Neda Mazaheri - Department of genetic, Faculity of Sciences, Shahid Chamran University of Ahvaz, Ahvaz- Department of Cell and Molecular Biology, Cell Science Research Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan
  Hamid Galedari - Department of genetic, Faculity of Sciences, Shahid Chamran University of Ahvaz, Ahvaz
  Maryam Peymani - Department of Cell and Molecular Biology, Cell Science Research Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan
  Kamran Ghaedi - Department of Cell and Molecular Biology, Cell Science Research Center, Royan Institute for Animal Biotechnology, ACECR, Isfahan- Department of Biology, School of Sciences, University of Isfahan, Isfahan

Abstract:

Inflammatory demyelinating diseases such as multiple sclerosis (MS) are characterized by damage to the myelin sheath surrounding neurons. Repair by remyelinating oligodendrocyte progenitor cells (OPCs) is a response to generation of inflammation. Apoptotic cell death of OPCs has been proposed to be the initial event in new lesion formation along with failure of remyelination. Reactive oxygen species such as hydrogen peroxide (H2O2) are one of the main apoptotic factors that cause oxidative stress. Here we have examined the exogenous H2O2 effects on induction of apoptosis in human OPCs culture . Method: Human OPCs were cultured and plated in 96-well plate at a density of 5000 cells/well. After 24h, The medium solution in each well was removed and cells were treated with serum free medium that contained a known concentration of H2O2 (50, 100, 200, 400, 800, 1600 and 3200 μM) diluted from a 9.8 M stock solution. The cells were incubated for 24 hr and MTS solution was added to each well for cell viability assay. After 4h incubation at 37º, The optical densities (A) at 492 nm were measured using an ELISA plate reader.

Keywords:

Inflammation, remyelination, Reactive oxygen species, oligodendrocyte progenitor cells (OPCs), apoptosis

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COI code: CIGS12_0417

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Mazaheri, Neda; Hamid Galedari; Maryam Peymani & Kamran Ghaedi, 2012, H(2)O(2) induced apoptosis in human oligodendrocyte progenitor cells (OPC(s)) culture, 12th Congress of Iranian Genetics Society, تهران, انجمن ژنتيك ايران, https://www.civilica.com/Paper-CIGS12-CIGS12_0417.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Mazaheri, Neda; Hamid Galedari; Maryam Peymani & Kamran Ghaedi, 2012)
Second and more: (Mazaheri; Galedari; Peymani & Ghaedi, 2012)
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Paper No.: 13703
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