Published in: 12th Congress of Iranian Genetics Society
COI code: CIGS12_1079
Paper Language: English
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Authors Characterization of stem cells isolated from peritoneal dialysed fluidTahereh Foroutan - Department of biology, Tarbiat Moallem University, Tehran, Iran
Abstract:The mesothelium, composed of monolayer of mesothelial cells (MCs) attached on a thin base membrane supported by a subserosal connective tissue, suggested as a dynamic structure that plays an important role in homeostasis, wound healing, fluid transport and inflammation. The introduction of peritoneal dialysis (PD) as a modality of renal replacement therapy has provoked much interest in the biology of the peritoneal MC. In the present study, we identified cells that express some defined markers of mesenchymal, hematopoietic and embryonic stem cells in human peritoneal mesothelial cells ( HPMCs).Materials and methods: In order to investigate the nature and plasticity of MCs isolated from peritoneal dialyzed fluid (PDF), cell populations from PDF of early stage non-peritonitis patients were screened for evaluating of dominant marker determination by cell culture, immunocytochemical, immunofluorescence and RT-PCR. Isolated cells were cultured and then in the first and 4th passages were studied for cytokeratine 18, HBME-1, CD34, CD38, CD90, CD452and alkaline phosphatase (ALP). Specific media for differentiation mesothelial cells to osteoblast cells were difined. Results: Our results were positive for some of defined markers and negative for the others. The formation of osteoblast like cells from mesothelial cells in specific media was confirmed by expression of osteopontin and ALP genes and alizarin red staining. Conclusion: It seems peritoneal MCs in culture possess immunocytochemical markers identical to mesothelial stem cells. This study suggests a multipotent plasticity of mesothelial cells.
Keywords:peritoneal dialysis, mesothelial cell, stem cell, characterization
COI code: CIGS12_1079
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Foroutan, Tahereh, 2012, Characterization of stem cells isolated from peritoneal dialysed fluid, 12th Congress of Iranian Genetics Society, تهران, انجمن ژنتيك ايران, https://www.civilica.com/Paper-CIGS12-CIGS12_1079.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
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