Published in: 12th Congress of Iranian Genetics Society
COI code: CIGS13_0102
Paper Language: English
How to Download This Paper
For Downloading the Fulltext of CIVILICA papers please visit the orginal Persian Section of website.
Authors A11337-Deletion Mutation Associated with Reduce Rate of the Nsun7 protein and Sperm Motility Defect in Asthenospermic Infertile MenNahid Khosronezhad -
Abasalt Hosseinzadeh Colagar -
Seyed Mohsen Mortazavi -
Esmat Ghalkhani -
Abstract:The Nsun7 gene, which encodes protein of putative methyltransferase Nsun7, has a role in sperm motility in mice. In humans, this gene is located on chromosome4. Since no similar studies have beenconducted in humans, so the aim of the present study was to investigate mutations of exon4 in the fertile (normospermic) and infertile asthenospermic (with reduced sperm motility) men. Semen samples were collected from the Omid and Fatemezahra IVF centers (Babol, Iran) and analyzed on the basis of World Health Organization (WHO) guidelines. Using general phenol–chloroform DNA extractionmethods. Exon4 was amplified using Sun-F and Sun-R primers. The exon4 PCR fragments were then dissected by PAGE to ensure that there were no additional bands, and then samples were sent to sequencing for further identification of possible mutations. Direct sequencing of polymerase chain reaction (PCR) products, along with their analysis, confirmed deletion mutation of adenine in location 11337 of Nsun7 gene in asthenospermic men. Comparison of normal and mutant protein structures of Nsun7 indicated that the through A11337-Deletion mutation was converted to stop codon and protein can be shorter than normal. Analysis of extracted proteins of the sperm showed that rate of the Nsun7 protein was reduced. Because Nsun7 gene products have a role in sperm motility, if a mutation occurred in exon4 of this gene, it would lead to impairment in activity of the protein and motility of sperm flagella, that causing sperm motility defect and infertility
Keywords:Nsun7 gene, Asthenospermic, Sperm motility, A11337-Deletion mutation, Male infertility, Deletion mutation
COI code: CIGS13_0102
how to cite to this paper:If you want to refer to this article in your research, you can easily use the following in the resources and references section:
Khosronezhad, Nahid; Abasalt Hosseinzadeh Colagar; Seyed Mohsen Mortazavi & Esmat Ghalkhani, 2014, A11337-Deletion Mutation Associated with Reduce Rate of the Nsun7 protein and Sperm Motility Defect in Asthenospermic Infertile Men, 12th Congress of Iranian Genetics Society, تهران, انجمن ژنتيك ايران, https://www.civilica.com/Paper-CIGS13-CIGS13_0102.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Khosronezhad, Nahid; Abasalt Hosseinzadeh Colagar; Seyed Mohsen Mortazavi & Esmat Ghalkhani, 2014)
Second and more: (Khosronezhad; Hosseinzadeh Colagar; Mortazavi & Ghalkhani, 2014)
For a complete overview of how to citation please review the following CIVILICA Guide (Citation)
Research Info Management
Export Citation info of this paper to research management softwares
New Related Papers
- Attenuation of inflammation genes expression by Juglans regia extract in an Osteoarthritis in vitro like-model.
- Identification of novel short tandem repeats based markers in the expressed sequence of Malus domestica Borkh.
- In silico study of anti-CCHFV effect of lactoferrin from different origin
- Optimization of DNA extraction from different tissues of a medicinal plant, Senecio vulgaris L.
- Design and construction of a new operator: A new attempt to improve protein expression under industrial condition
The Above articles are recently indexed in the related subjects
Iran Scientific Advertisment Netword
Share this paper
WHAT IS COI?
COI is a national code dedicated to all Iranian Conference and Journal Papers. the COI of each paper can be verified online.