Published in: 12th Congress of Iranian Genetics Society
COI code: CIGS13_0242
Paper Language: English
How to Download This Paper
For Downloading the Fulltext of CIVILICA papers please visit the orginal Persian Section of website.
Authors Design of non-chromatographic purification-vectors for optimization of purification process of pharmaceutical proteins and industrial enzymesRahele Norouzi - Division of Genetics, Department of Biology, Faculty of Science, University of Isfahan, Isfahan, IR Iran
Zohreh Hojati Najaf abadi - Division of Genetics, Department of Biology, Faculty of Science, University of Isfahan, Isfahan, IR Iran
Abstract:Development of protein expression systems cause production of every peptides in at least one host cell. Finally, for the next examinations, the produced protein should be purify. Purification tags are powerful tools that can be used in this order. The desired protein purification can be facilitate through the integration of its genetic sequence with a tag. This tag plays role as a mediator for protein separation from other cell contents.Object: One of the limitations of present approach is unwanted effects of purification tag on natural activity, physicochemical characteristics and next applications of protein. So, tag isolation from target protein after purification should be considered as an essential step. Doing this process with the help of dedicated endopeptidase lead to time and cost consumption and need to a further enzyme separation step. So, the proper replacement for the enzymatic tag removal methods is veryimpressive. Method: For eliminating expensive enzymes, the main focus of this study is to design purification vectors based on mutant selfsplicing mini-inteins. Furthermore, in order to omission of the need for expensive chromatographic columns, separating twocandidate proteins, interferon beta and somatropin from other cell contents will be mediated via designed ELP sequence inour vectors which is another advantage of present purification constructions. Purify percentage examination of proteins via confirmation test such as SDS-PAGE and Western-blot will be significant. Results: Construction of presented vectors provide a wide range of industrial and pharmaceutical protein purification potential. This topic can make them appropriate options for commercial protein purification kits design
Keywords:purification tags, self-splicing tags, intein, ELP
COI code: CIGS13_0242
how to cite to this paper:If you want to refer to this article in your research, you can easily use the following in the resources and references section:
Norouzi, Rahele & Zohreh Hojati Najaf abadi, 2014, Design of non-chromatographic purification-vectors for optimization of purification process of pharmaceutical proteins and industrial enzymes, 12th Congress of Iranian Genetics Society, تهران, انجمن ژنتيك ايران, https://www.civilica.com/Paper-CIGS13-CIGS13_0242.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Norouzi, Rahele & Zohreh Hojati Najaf abadi, 2014)
Second and more: (Norouzi & Hojati Najaf abadi, 2014)
For a complete overview of how to citation please review the following CIVILICA Guide (Citation)
The University/Research Center Information:
Type: state university
Paper No.: 13235
in University Ranking and Scientometrics the Iranian universities and research centers are evaluated based on scientific papers.
Research Info Management
Export Citation info of this paper to research management softwares
New Related Papers
- Composition, distribution and economic importance of insect pests of prioritized aromatic plants in some growing of Ethiopia
- Quantitative genetic analysis of Dromedary camel (Camelus dromedaris) under pastoral management in central desert of Iran
- Bimolecular identification of M.ovinpeumoniae from nasal swab samples of sheep from various districts of Balochistan, Pakistan
- Salt and water stress of ACCase herbicides resistant and susceptible populations of rigid ryegrass (Lolium rigidum)
- A Monte Carlo study on Photoneutron Spectrum around Elekta SL75/25 18 MV linear accelerator
The Above articles are recently indexed in the related subjects
Iran Scientific Advertisment Netword
Share this paper
WHAT IS COI?
COI is a national code dedicated to all Iranian Conference and Journal Papers. the COI of each paper can be verified online.