Design of non-chromatographic purification-vectors for optimization of purification process of pharmaceutical proteins and industrial enzymes

Development of protein expression systems cause production of every peptides in at least one host cell. Finally, for the next examinations, the produced protein should be purify. Purification tags are powerful tools that can be used in this order. The desired protein purification can be facilitate through the integration of its genetic sequence with a tag. This tag plays role as a mediator for protein separation from other cell contents.Object: One of the limitations of present approach is unwanted effects of purification tag on natural activity, physicochemical characteristics and next applications of protein. So, tag isolation from target protein after purification should be considered as an essential step. Doing this process with the help of dedicated endopeptidase lead to time and cost consumption and need to a further enzyme separation step. So, the proper replacement for the enzymatic tag removal methods is veryimpressive. Method: For eliminating expensive enzymes, the main focus of this study is to design purification vectors based on mutant selfsplicing mini-inteins. Furthermore, in order to omission of the need for expensive chromatographic columns, separating twocandidate proteins, interferon beta and somatropin from other cell contents will be mediated via designed ELP sequence inour vectors which is another advantage of present purification constructions. Purify percentage examination of proteins via confirmation test such as SDS-PAGE and Western-blot will be significant. Results: Construction of presented vectors provide a wide range of industrial and pharmaceutical protein purification potential. This topic can make them appropriate options for commercial protein purification kits design

Keywords:

purification tags , self-splicing tags , intein , ELP