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title

Comet assay, a reliable screening tool for SCID radiosensitivity

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Year: 2014
COI code: CIGS13_0274
Paper Language: English

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Authors Comet assay, a reliable screening tool for SCID radiosensitivity

  Marzie Mahmoodi - Cell and Molecular Biology Department,faculty of Biological Sciences, Science and Research University(SRBIAU),Tehran, Iran,Department of medical genetics, Iran University of medical sciences. Tehran ,Iran, Department of human genetics, Tehran University o
  Shahram Teimourian - Department of medical genetics, Iran University of medical sciences. Tehran ,Iran, Department of human genetics, Tehran University of medical sciences. Tehran ,Iran, Pediatrics Infectious Diseases Research Center, Department of Infectious Disease, School

Abstract:

Severe Combined Immunodeficiency Disease (SCID) is the most serious and rare but important primary immunodeficiency disorder. The defining characteristic of SCID is the absence of T cells andlack of functional B cells. This disease can be inherited in two forms of X-dependent and autosomalrecessive. Patients with SCID are treated with BMT. Imunocompetent NK cells in radiosensitive SCID patients pose a significant barrier to engraftment of HLA-mismatched donor stem cells as high-dose of chemotherapy or radiotherapy is applied to suppress NK cells to prevent GVHD. Radiosensitive SCID patients need HLA match donors. Method In this study, fibroblast cells of children suspected to SCID were cultured and were used in cometassay technique followed by comet score analysis by software freeware v1.5. and compared with colonogenic assay Result In this study, fibroblast cells of children suspected to SCID showed rate of DNA damage repair by colonogenic assay relatively the same as alkalin comet assay (The Single Cell Gel Electrophoresisassay) Discusion This technique is fast, inexpensive and accurate for radiosensitivity assay in children suspected to SCID disease relative to colonogenic assay .

Keywords:

SCID, comet assay, clonogenic assay, ARTEMIS

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COI code: CIGS13_0274

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Mahmoodi, Marzie & Shahram Teimourian, 2014, Comet assay, a reliable screening tool for SCID radiosensitivity, 12th Congress of Iranian Genetics Society, تهران, انجمن ژنتيك ايران, https://www.civilica.com/Paper-CIGS13-CIGS13_0274.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
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Type: Medical University
Paper No.: 3020
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