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Detection of LT toxin in Enterotoxigenic Escherichia coli by PCR-ELISA technique

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Year: 2014
COI code: CIGS13_0306
Paper Language: English

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Authors Detection of LT toxin in Enterotoxigenic Escherichia coli by PCR-ELISA technique

Pantea Esfandiari - Department of Biology, Damghan Azad University, Damghan, Iran. Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran.
  Jafar Amani - Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
  Abbas Ali Imani fouladi - Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Mohammad Mahdi Forghanifard - Department of Biology, Damghan Azad University, Damghan, Iran.

Abstract:

Background: ETEC is the most common agent which causes diarrhea. In this research PCR-ELISA method is used for detecting ETEC. Materials and methods: ETEC is colonized along the cells and then producing heat-labile (LT) and heat-stable enterotoxigenic at the end causes diarrhea. In this way DIG-labeled PCR products were bounded to streptoavidin-coatedwells of a microtiter plate and detected by anti-DIG–peroxidase conjugate. Internal biotin labeled probe was designed forLT gene and detected with streptavidin. Result: In this research by the use of specified primer and PCR-ELISA technique, LT toxin of ETEC was analyzed. Resultsshowed that this method was the fastest, exactest and the most sensitive way for diagnosing bacterial.Conclusion: various methods of PCR are used for diagnosing different spices of ETEC and also the most common methods; here are the uses of the gel electrophoresis, hybridization and colorization by Ethidium bromide. PCR-ELISA is a suitablesubstitute for all the above aspects because it's quite sensitive specific and rapid way for detection of ETEC

Keywords:

Enterotoxigenic Escherichia coli, PCR-ELISA, Heat-labile enterotoxin

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COI code: CIGS13_0306

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Esfandiari, Pantea; Jafar Amani; Abbas Ali Imani fouladi & Mohammad Mahdi Forghanifard, 2014, Detection of LT toxin in Enterotoxigenic Escherichia coli by PCR-ELISA technique, 12th Congress of Iranian Genetics Society, تهران, انجمن ژنتيك ايران, https://www.civilica.com/Paper-CIGS13-CIGS13_0306.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Esfandiari, Pantea; Jafar Amani; Abbas Ali Imani fouladi & Mohammad Mahdi Forghanifard, 2014)
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