Published in: 12th Congress of Iranian Genetics Society
COI code: CIGS13_0325
Paper Language: English
How to Download This Paper
For Downloading the Fulltext of CIVILICA papers please visit the orginal Persian Section of website.
Authors Cytogenetic and Molecular Cytogenetic Study in Chronic Myelogenous Leukemia PatientsSoheila MANAFLOUYAN KHAJEHMARJANY - Department of Cellular & Molecular Biology, Islamic Azad University, Ahar Branch, Islamic republicof Iran Dr. Rahmani Medical Genetic Lab, Tabriz, Islamic republic of Iran
Seyed Ali RAHMANI - Corresponding Address: P.O.Box: 5451116714, Department of Cellular & Molecular Biology, Islamic Azad University, Ahar Branch, Iran
Seyed Hadi CHAVOSHI - M.D Department of Hematology & Oncology Research Center -Tabriz University of Medical Sciences, Islamic republic of Iran Emam Reza Hospital, Tabriz, Islamic republic of Iran
Ali ESFAHANI - Department of Hematology & Oncology Research Center -Tabriz University of Medical Sciences, Islamic republic of Iran Emam Reza Hospital, Tabriz, Islamic republic of Iran
Abstract:BACKGROUND: Chronic myeloid leukemia (CML) is a myeloproliferative disease. The cytogenetic hallmark of CML is Philadelphia chromosome (Ph). This study aimed to diagnose assumed CML patients, to monitor CML patients under therapy on the bone marrow (BM) and peripheral blood (PB) samples by cytogenetic and fluorescence in situ hybridization(FISH), and also to compare the results on both specimens. MATERIALS & METHODS: Chromosome analysis was performed on 60 suspected CML patients by G-banding and FISH on the BM and PB specimens. RESULTS: Of 90 samples being analyzed on 60 patients, 25 (41.66%) were Ph+ by karyotyping, whilst five cases of BMsamples were not analyzable. In comparison between 25 BM and PB samples by karyotyping, respectively 15 (60%) and 10 (40%) were ph+. The comparison of FISH and karyotyping in 30 patients, respectively showed that 9 (30%) and 8 (26.66%) were Ph+, and only 18.18% of Ph positive patients showed atypical patterns. In comparison between BM-cytogenetic and PB I-FISH, BM–cytogenetic was more reliable than PB I-FISH to detect Ph.CONCLUSION: Our data demonstrate FISH analysis is a rapid, reliable and sensitive technique. The comparison between BM and PB showed BM can't replace by PB, even in detecting by FISH
Keywords:CML, FISH, Philadelphia Chromosome (Ph), Cytogenetic
COI code: CIGS13_0325
how to cite to this paper:If you want to refer to this article in your research, you can easily use the following in the resources and references section:
MANAFLOUYAN KHAJEHMARJANY, Soheila; Seyed Ali RAHMANI; Seyed Hadi CHAVOSHI & Ali ESFAHANI, 2014, Cytogenetic and Molecular Cytogenetic Study in Chronic Myelogenous Leukemia Patients, 12th Congress of Iranian Genetics Society, تهران, انجمن ژنتيك ايران, https://www.civilica.com/Paper-CIGS13-CIGS13_0325.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (MANAFLOUYAN KHAJEHMARJANY, Soheila; Seyed Ali RAHMANI; Seyed Hadi CHAVOSHI & Ali ESFAHANI, 2014)
Second and more: (MANAFLOUYAN KHAJEHMARJANY; RAHMANI; CHAVOSHI & ESFAHANI, 2014)
For a complete overview of how to citation please review the following CIVILICA Guide (Citation)
The University/Research Center Information:
Type: Azad University
Paper No.: 2252
in University Ranking and Scientometrics the Iranian universities and research centers are evaluated based on scientific papers.
Research Info Management
Export Citation info of this paper to research management softwares
New Related Papers
- Attenuation of inflammation genes expression by Juglans regia extract in an Osteoarthritis in vitro like-model.
- Identification of novel short tandem repeats based markers in the expressed sequence of Malus domestica Borkh.
- In silico study of anti-CCHFV effect of lactoferrin from different origin
- Optimization of DNA extraction from different tissues of a medicinal plant, Senecio vulgaris L.
- Design and construction of a new operator: A new attempt to improve protein expression under industrial condition
The Above articles are recently indexed in the related subjects
Iran Scientific Advertisment Netword
Share this paper
WHAT IS COI?
COI is a national code dedicated to all Iranian Conference and Journal Papers. the COI of each paper can be verified online.