CIVILICA We Respect the Science
Publisher of Iranian Journals and Conference Proceedings
Paper
title

Prediction of three dimensional structure of Kruppel-like factor 14 protein

Credit to Download: 0 | Page Numbers 1 | Abstract Views: 571
Year: 2014
COI code: CIGS13_0477
Paper Language: English

How to Download This Paper

For Downloading the Fulltext of CIVILICA papers please visit the orginal Persian Section of website.

Authors Prediction of three dimensional structure of Kruppel-like factor 14 protein

Ensieh Shahvazian - Yazd Medical University of Shahid Sadoghi, International Campus, Genetic department
Mohammad bagher Mahmoudi - Yazd Medical University of Shahid Sadoghi, Genetic department
Masoud Mirzaei - Yazd Medical University of Shahid Sadoghi, Epidemiology department
Ehsan Farashahi Yazd - Yazd Medical University of Shahid Sadoghi, Genetic department

Abstract:

KLF14 (Kruppel-like factor 14) is a member of the Kruppel-like family of transcription factors. KLF14 acts as a master trans-regulator of adipose gene expression. It is located on 7q32.3 and is an intronless gene. Many GWAs have paid attention to this gene since 2010, due to its important participation in metabolic disease.Aim: Crystallography studies have not been accomplished for it yet. So in This study we attempted to predict 3D structure ofKLF14 protein via in silico approaches. Material method: Protein sequence served as a query for BLAST search against Protein Database. This study was conducted to develop 3Dmodels of KLF14 by invoking various in silico methods such as homology modeling, threading, and combination of them aswell as ab initio including; I-TASSER, LOMETS, LOOP, MODELLER, PHYRE2, (PS)2-v2, ESyPred3D, SPARKS-X Software. Result: By comparing and analyzing the Qmean and z-score, energy level and Ramachadran plot, we selected 5 best structures with the highest favored region in Ramachadran plot (higher than 96.9%) and refine them with MODREFINER software. The best 3D structure was predict by SPARKS-X employing threading algorithm with 99.4% amino acids in favored region. Conclusion: Based on the importance of this gene in several multifactorial diseases and also because of the unique characteristics of the gene which amplify the effect of even single nucleotide changes in all parts of the gene, the results of our study can be a guide for future functional analysis studies.

Keywords:

KLF14, 3D structure, adipose tissue, homology modeling

Perma Link

https://www.civilica.com/Paper-CIGS13-CIGS13_0477.html
COI code: CIGS13_0477

how to cite to this paper:

If you want to refer to this article in your research, you can easily use the following in the resources and references section:
Shahvazian, Ensieh; Mohammad bagher Mahmoudi; Masoud Mirzaei & Ehsan Farashahi Yazd, 2014, Prediction of three dimensional structure of Kruppel-like factor 14 protein, 12th Congress of Iranian Genetics Society, تهران, انجمن ژنتيك ايران, https://www.civilica.com/Paper-CIGS13-CIGS13_0477.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Shahvazian, Ensieh; Mohammad bagher Mahmoudi; Masoud Mirzaei & Ehsan Farashahi Yazd, 2014)
Second and more: (Shahvazian; Mahmoudi; Mirzaei & Farashahi Yazd, 2014)
For a complete overview of how to citation please review the following CIVILICA Guide (Citation)

Research Info Management

Export Citation info of this paper to research management softwares

New Related Papers

Iran Scientific Advertisment Netword

Share this paper

WHAT IS COI?

COI is a national code dedicated to all Iranian Conference and Journal Papers. the COI of each paper can be verified online.