Published in: 12th Congress of Iranian Genetics Society
COI code: CIGS13_0899
Paper Language: English
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Authors Genetic Engineered Interferonβ proteins expressed in Escherichia coliMahnaz Sadeghi - Departments of Biology, Higher Education Institute of Rab-Rashid, Tabriz, Iran,
Safar Farajnia - Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Fereidoun Mahboudi - Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
Bahram Baghban - Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran
Abstract:The new generation of biologial products are largely the result of genetic engineering. The qualitative and quantitative demand for recombinant proteins is steadily increasing. Molecular biologis are constantly challenged by the need to improve and optimise the existing expression system, and also develop novel approaches to face the demands of producingthe complex proteins of tomorrow. Technologies based on genetic recombination are paving the way towards thetherapeutic usage of complex molecules. These proteins are proving useful in numerous areas; from the treatment multiplesclorosis to complex cancer immunotherapies targeting effector molecules. One of the strategies used by researchers in this field involves using different signal peptides to increas the expression level . In this study we compare the effect of two different signal peptide pelB and OmpA on the solubility and expression of recombinant human IFNβ in E.coli Expression system. Based on this study it was concluded that the choice of signal peptide sequence has a strong impact on both the translocation and the expression level of recombinant IFNβ.
Keywords:Genetic engineer, IFNβ, Multiple Sclerosis, recombinant proteins
COI code: CIGS13_0899
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Sadeghi, Mahnaz; Safar Farajnia; Fereidoun Mahboudi & Bahram Baghban, 2014, Genetic Engineered Interferonβ proteins expressed in Escherichia coli, 12th Congress of Iranian Genetics Society, تهران, انجمن ژنتيك ايران, https://www.civilica.com/Paper-CIGS13-CIGS13_0899.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Sadeghi, Mahnaz; Safar Farajnia; Fereidoun Mahboudi & Bahram Baghban, 2014)
Second and more: (Sadeghi; Farajnia; Mahboudi & Baghban, 2014)
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