COI code: CIGS15_002
Paper Language: English
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Authors Attenuation of inflammation genes expression by Juglans regia extract in an Osteoarthritis in vitro like-model.e mohamadi - msc. Genetics & Biology teacher at education & training ministry,Tehran,Iran
eqbal mohamadi - m.sc Genetics & Biology teacher at education & training ministry,Tehran,Iran
Abstract:Today, the use of herbal medicine in common disease control and treatment, such as inflammatory diseases; is increasing. The present study aimed to assess anti-inflammatory effects of Juglans regia extract in an osteoarthritis-like model on synoviocytes cells cultured from Holstein cows. the extract cytotoxicity was determined as 1mg/dL by MTT, LDH and SDH assay. Then LC50 was determined as 1µg/ml. The pre-inflammatory cytokines TNF-α, IL-18 and IL-1β genes expression treated with NSAIDs, Steroids and the extract was assessed in groups as followings; Cell, Cell+LPS, Cell+LPS+NSAIDs, Cell+LPS+Steroids and Cell+LPS+Juglans regia extract. Then using real-time PCR technique, analyzed with Pffafl, F static and ANOVA methods and contrasting with REST 2009 software. The results showed down-regulating expression of the inflammatory genes (p<.05), due to Juglans regia extract and anti-inflammatory effects of the extraction. In the present study, it proved that effects of Persian walnut extract on synoviocytes decreased the expression of genes involved in inflammation 42.37%,47.53% and 42.81% respectively for TNF-α, IL-1β and IL-18; contrasting to control group. So Persian walnut ingredients can be used as an alternative treatment and it can be offered as well as an effective recommended.
Keywords:anti-inflammatory effects, Juglans regia extract, cytokines, synoviocytes, gene expression, qPCR.
COI code: CIGS15_002
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mohamadi, e & eqbal mohamadi, 2018, Attenuation of inflammation genes expression by Juglans regia extract in an Osteoarthritis in vitro like-model., The Third International and 15th National Genetics Congress, تهران, انجمن علمي ژنتيك ايران, https://www.civilica.com/Paper-CIGS15-CIGS15_002.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (mohamadi, e & eqbal mohamadi, 2018)
Second and more: (mohamadi & mohamadi, 2018)
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