Evaluation of genetic diversity in Capsicum spp as revealed by FARS markers

One of the most important herbs in the pharmaceutical, spice is pepper. Nowadays, the use of molecular markers to study genetic diversity .The existence of polymorphism in plant species is one of the most important tools for gene identification, genome screening and development of genetic maps. For detection of such polymorphism based on PCR, are used two primers, and in some other methods such as RAPD, DAF and AP-PCR, only one primer is used. However, in later cases nucleotide combination of such primers is completely random and their physical location on genome is unclear. On the other hand, the primers produce large number of bands with low repeatability. One alternative is to use sequences that provide the elements necessary for their synthesis. Among these sequences, one can refer to the palindromic regions of the genome, which have a short reverse sequence on both ends. This short reverse sequence can be used in the PCR reaction as a primer and can cause the palindromic region to be synthesized between the two reverse sequences. This region is multiplied by this single primer, called the (FARS) region amplified. It could identified in the plant sequenced genomes using bioinformatic tools, and single primers needed for PCR were synthesized and tested. Using the specific FARS primers it could be confirmed the possibility of PCR- based amplification of short , relatively long and long palindromic regions and the marker system FARS could produce co-dominant bands which is suitable for detection of polymorphism between different genotypes

Keywords:

Molecular marker , Polymerase chain reaction , FARS , pepper , palindromic