Multilocus sequence typing) MLST( detection of diarragenic Escherichia colis pathotypes

Escherichia coli (E. coli) are usually a harmless commensal normal inhabitant of gut flora in humans. Some strains have the capacity to cause disease in human and animals by specific pathogenic mechanisms that in some cases can cause to infection resulting to death. Serotyping is a method for classification of E. coli that has been developed into standardized procedures. This method is one of the times and cost consuming that needs experts and antisera. Typing can be done only in some of the laboratories. In fact, typing methods can be used for strains classification by different methods. Despite serogrouping and phenotyping there are usual genotyping methods such as RFLP and pulsed-field gel electrophoresis (PFGE). Multilocus Sequence Typing (MLST (method can also use for strain discrimination. In this study, 50 E. coli strains from National E. coli Reference Laboratory were used. DNA from classified strain types was isolated from cultured strains and assessed.Multilocus Sequence Typing (MLST) with 7 housekeeping genes: adk (adenylate kinase ), fum C (fumarate hydratase), gyrB ( DNA gyrase), icd (isocitrate dehydrogenase), mdh (malate dehydrogenase), purA (adenylosuccinate synthetase), and recA (adenosine triphosphate/ guanosine triphosphate binding motif) used. These primers suggested by Pasteur institute (http://www.pasteur.fr/recherche/genopole/PF8/mlst/EColi.html).Rapid detection of bacterial pathogenic diseases in this study was done by MLST which is a standard method for clonal detection and classification. Furthermore, the digitalization of MLST data and web-accessible databases can be done and it also could rapidly contribute to the clonal distribution of infectious disease agents in future, specifically in Reference Laboratories.