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Analysis of Promoter Methylation, Polymorphism and Expression Profile of Cytotoxic T-Lymphocyte-Associated Antigen-4 in Patients with lupus

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Year: 2018
COI code: CIGS15_140
Paper Language: English

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Authors Analysis of Promoter Methylation, Polymorphism and Expression Profile of Cytotoxic T-Lymphocyte-Associated Antigen-4 in Patients with lupus

  s Atighi - Department of Genetics, Faculty of Sciences, Sistan and Baluchestan University, Zahedan, Iran
  d m Kordi tamandani - Department of Genetics, Faculty of Sciences, Sistan and Baluchestan University, Zahedan, Iran
  sh nosrat zehi - Department of Genetics, Faculty of Sciences, Sistan and Baluchestan University, Zahedan, Iran

Abstract:

Background: Systemic lupus erythematous (SLE) is an autoimmune disease with unknown etiology, in which autoantibodies directly contribute to the destruction of organs such as kidneys, joints, and skin.The cytotoxic T lymphocyte T-4 antigens (CTLA4) play an important role in inhibition the activity of T cells and thus preventing autoimmune disorders like lupus.Material and method: We isolated genomic DNA from peripheral blood of 50 individuals with SLE and 50 control subjects. Analysis of CTLA4 gene polymorphisms in polymorphic sites -318(CT) and +49(AG)was done by Tetra-ARMS-PCR. Methylation-specific polymerase chain reaction (MS-PCR) used to estimate promoter hyper methylation of the CTLA4 gene. we investigated CTLA4 mRNA levels in 30 blood samples from cases and healthy controls using real-time reverse transcription PCR.Result: Promoter methylation changes of CTLA4 gene was remarkably different in patients with lupus in comparison with healthy controls (OR= 0.48; 95% CI= 0.1959, 1.202; P-value= 0.005). However, gene expression level of CTLA4 were not statistically different in patients and healthy controls (Mean ± SD: 1.24 ± 0.827 and 1.071 ± 0.736; P-value = 0.55).Conclusion: this epigenetic study may give us an overview of the role of promoter methylation in the pathogenesis of SLE. However, further studies on more populations with larger sample sizes need to be done to verify this hypothesis in the future.

Keywords:

Systemic lupus erythematous, Promoter Methylation, Polymorphism, Gene Expression

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COI code: CIGS15_140

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Atighi, s; d m Kordi tamandani & sh nosrat zehi, 2018, Analysis of Promoter Methylation, Polymorphism and Expression Profile of Cytotoxic T-Lymphocyte-Associated Antigen-4 in Patients with lupus, The Third International and 15th National Genetics Congress, تهران, انجمن علمي ژنتيك ايران, https://www.civilica.com/Paper-CIGS15-CIGS15_140.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
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Type: state university
Paper No.: 9135
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