Determining the ABO & Rh blood groups using the SNaPshot Technique

The determination of the ABO and Rh blood type is one of the tests used to identify individuals in forensic science. Determining this type of blood group is currently performed by testing the antigens A and B on the red blood cell membrane in serological tests in clinical laboratories. Serologic methods are one of the important techniques for determining the blood type, which, if performed, requires some blood; in many cases, the amount of biological samples with high resolution is very limited and insignificant or there is no blood sample at all, and the sample is related to Other tissues, in this case genotypic examination for the diagnosis of blood group states is a valuable method in forensic medicine, especially when routine phenotypic examination is not feasible. The specific combination of SNPs in the ABO locus on chromosome 9 and Rh on chromosome 1 can lead to the identification of various types of ABO and Rh blood types from all body cells such as blood, bone and similar ones. This study was performed to determine the blood groups of ABO and Rh. At First, DNA was purified from blood and bone samples. Then, SNaPshot method was used to determine the type of SNPs that determines the blood groups, then the blood samples were finally confirmed by serological tests.

Keywords:

ABO & Rh blood groups , identification , SNP , PCR , SNaPshot