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Design and construction of a new operator: A new attempt to improve protein expression under industrial condition

Credit to Download: 1 | Page Numbers 11 | Abstract Views: 51
Year: 2018
COI code: CIGS15_229
Paper Language: English

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Authors Design and construction of a new operator: A new attempt to improve protein expression under industrial condition

Mohammad Saba Yousef Mardoukhi - Department of Genetics, Faculty of Science, University of Shahrekord, Shahrekord, Iran.
Ali Mohammad Ahadi - Department of Genetics, Faculty of Science, University of Shahrekord, Shahrekord, Iran.
Hoda AYAT - Department of Genetics, Faculty of Science, University of Shahrekord, Shahrekord, Iran.
Seyed Shahriar Arab - Department of Biophysics, Faculty of Biological Science, Tarbiat Modarres University, Tehran, Iran.

Abstract:

SOS response system is one of the most studied cellular systems in many bacterial species. Almost more than forty-independent SOS genes are engaged in function of SOS system. Encoded proteins participate in some process like replication, mutagenesis, repair, and subsequently, response to any intra/extra-cellular stress that can harm to DNA structure or DNA replication. When there is no any inductive stress (normal growth conditions), expression of SOS genes is at a basic level, after induction, the level of expression exponentially rises. Induction of SOS response system contains more than 40-independent SOS genes that encode proteins engaged in the protection, repair, replication, mutagenesis and DNA metabolism. Under normal growth conditions, SOS genes are expressed at a basic level, as a result of induction, the expression significantly increased. In this study, we designed and analyzed a derivative operator compatible for a broad range of temperatures. In silico analysis of this study showed a very interactive construct that can attract effectively the lexA repressor. This study is a primary modelling study for creation of a new generation of vectors applicable in the industrial biotechnology.

Keywords:

SOS, operator, interaction, LexA

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COI code: CIGS15_229

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Saba Yousef Mardoukhi, Mohammad; Ali Mohammad Ahadi; Hoda AYAT & Seyed Shahriar Arab, 2018, Design and construction of a new operator: A new attempt to improve protein expression under industrial condition, The Third International and 15th National Genetics Congress, تهران, انجمن علمي ژنتيك ايران, https://www.civilica.com/Paper-CIGS15-CIGS15_229.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Saba Yousef Mardoukhi, Mohammad; Ali Mohammad Ahadi; Hoda AYAT & Seyed Shahriar Arab, 2018)
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