Expression analysis of inflammatory gene VEGFA and miR-29a in coronary artery Ectasia and Coronary artery disease in patients of southeast of Iran-Kerman

Publish Year: 1397
نوع سند: مقاله کنفرانسی
زبان: English
View: 358

نسخه کامل این Paper ارائه نشده است و در دسترس نمی باشد

  • Certificate
  • من نویسنده این مقاله هستم

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این Paper:

شناسه ملی سند علمی:

CIGS15_239

تاریخ نمایه سازی: 13 بهمن 1398

Abstract:

Coronary artery ectasia (CAE) is defined as dilatation of the coronary artery 1.5 times greater than that of an adjacent normal segment. Pathogenetic mechanisms of CAE are still unknown. Vascular Endothelial Growth Factor (VEGF), is a major regulator of angiogenesis, and may play a role in CAE. Also, MicroRNA-29a is known to be involved in the down-regulation of VEGF. The aims of this study were to clarify the significance of VEGF and MicroRNA-29a genes expression in relation to CAE compare to CAD in Southeast of Iran (Kerman population). Methods:58 subjects were enrolled in our study (34 CAE and 24 CAD patients), these were compared with 25 control subjects. The quantitative real-time reverse transcriptase PCR was used for evaluation of genes transcripts.Blood were collected in EDTA tubes and the Peripheral Blood Mononuclear Cells (PBMCs) were cultured for 7 days, total RNA was extracted from PBMCs reverse transcribed into cDNA, amplified and quantitated by SYBR Green detection.Result:In our study, expression of VEGF was higher in CAE compared with CAD patients and control but not significant, and the expression of Mir29a was lower in CAE than CAD patients Conclusion:MicroRNA-29 expression has upregulated in our CAD subjects and down regulated VEGF which follows the other investigator’s result. In CAE, the Upregulation of mir29a had no effect in VEGF expression.

Authors

Mozhgan Sheikhoeslami,

Physiology Research Center, Kerman university of Medical Sciences

Saeideh Jafarinejad,

Physiology Research Center, Kerman university of Medical Sciences

Azadeh Khajoee

Physiology Research Center, Kerman university of Medical Sciences

Mariam Shahrokhi

Physiology Research Center, Kerman university of Medical Sciences