Identification of potential miRNAs involved in colorectal cancer progression through DNA Repair system;A comprehensive meta-analysis

Colorectal cancer (CRC) is known as the third prevalent cancer and the third leading cause of cancer mortality worldwide.In recent years, many domestic and foreign research have carried out a number of studies on the role of microRNAs in colorectal cancer.MicroRNAs (miRNAs) are a class of endogenous, small (17-25 nucleotides),non-coding ,single-stranded and highly conserved RNA molecules that regulate gene expression at post-transcription level.miRNAs might function as oncogenes or tumor suppressors in the process of tumorgenesis depending on the tumor microenvironment and the targets they regulate.In this study,899 miRNAs from miRNA Pathway Dictionary Database (miRPathDB) which are colorectal cancer-specific were analysed. We have used different kinds of bioinformatics tools such as Targetscan, miRwalk, miRTar, miRTarBase, miRfocus to predict target genes of the selected miRNAs, the target genes list were narrowed down by searching the data from KEGG database to identify DNA Repair–specific genes. we also have used ym500v3, dbDEMC, miRBase, miRIAD for analysing Fold change ,expression and sequences of our desired miRNAs. Finally we checked Pubmed and google Scholar to identify eligible studies.Different miRNAs such as Has-miR-940,Has-miR-7-5p,Has-miR-432-5p and Has-miR-186-3p were detected by combining data from all above databases. So, our study suggest miRNAs with potential role in colorectal cancer.

Keywords:

Colorectal cancer , miRNAs , Bioinformatics tools