CIVILICA We Respect the Science
Publisher of Iranian Journals and Conference Proceedings
Paper
title

A rapid, Simple Genomic DNA Extraction from Yeasts for Large Scale Genetic Analyses

Credit to Download: 1 | Page Numbers 5 | Abstract Views: 49
Year: 2018
COI code: CIGS15_307
Paper Language: English

How to Download This Paper

For Downloading the Fulltext of CIVILICA papers please visit the orginal Persian Section of website.

Authors A rapid, Simple Genomic DNA Extraction from Yeasts for Large Scale Genetic Analyses

  Faezeh Ajorloo - Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), P.O. Box 33535111, Tehran, Iran
  Mohsen Vaez - Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), P.O. Box 33535111, Tehran, Iran
  Jafar Hemmat - Department of Biotechnology, Iranian Research Organization for Science and Technology (IROST), P.O. Box 33535111, Tehran, Iran

Abstract:

DNA extraction methods usually rely on time-consuming procedures that may involve handling hazardous compounds or using expensive kit. Here, we have developed a rapid genomic DNA extraction technique from yeasts and applied it for amplification of genomic DNA using gene specific primers. DNA extraction was evaluated at different stages from initial step to final pure DNA. In addition to final pure DNA of each sample which runs on an agarose gel to check the quantity and efficiency of whole yield, the extracts at different stages of extraction procedure were qualitatively examined and evaluated using gene amplification in a PCR reaction for minimal time consumption and lack of PCR inhibitors. The results showed that the earliest step for successful gene amplification was immediate after one hour incubation in 65 oC with extraction buffer. The obtained DNA was validated with different sets of primers for DNA amplification. The PCR products from each sliced bands were purified and sequenced. This evaluation showed that the presented method is a reliable, rapid and simple procedure with minimal equipment and resources requirements which is also environmentally friendly in absence of toxic chemicals for a cost efficient DNA extraction especially for large number of samples in less than one hour for sufficient genomic DNA for different purposes from yeasts for large scale genetic analyses including downstream PCR-based genetic analysis.

Keywords:

Perma Link

https://www.civilica.com/Paper-CIGS15-CIGS15_307.html
COI code: CIGS15_307

how to cite to this paper:

If you want to refer to this article in your research, you can easily use the following in the resources and references section:
Ajorloo, Faezeh; Mohsen Vaez & Jafar Hemmat, 2018, A rapid, Simple Genomic DNA Extraction from Yeasts for Large Scale Genetic Analyses, The Third International and 15th National Genetics Congress, تهران, انجمن علمي ژنتيك ايران, https://www.civilica.com/Paper-CIGS15-CIGS15_307.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Ajorloo, Faezeh; Mohsen Vaez & Jafar Hemmat, 2018)
Second and more: (Ajorloo; Vaez & Hemmat, 2018)
For a complete overview of how to citation please review the following CIVILICA Guide (Citation)

Scientometrics

The University/Research Center Information:
in University Ranking and Scientometrics the Iranian universities and research centers are evaluated based on scientific papers.

Research Info Management

Export Citation info of this paper to research management softwares

New Related Papers

Iran Scientific Advertisment Netword

Share this paper

WHAT IS COI?

COI is a national code dedicated to all Iranian Conference and Journal Papers. the COI of each paper can be verified online.