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title

Allelic variation of GSP gene in some wild wheat with A genome

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Year: 2018
COI code: CIGS15_326
Paper Language: English

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Authors Allelic variation of GSP gene in some wild wheat with A genome

  Maryam Mansourian - Agronomy and plant breeding department, faculty of agriculture, Ilam University, Ilam, Iran
  Arash Fazeli - Agronomy and plant breeding department, faculty of agriculture, Ilam University, Ilam, Iran
  Hamid Hosseinan Khoshro - Agronomy and plant breeding department, faculty of agriculture, Ilam University, Ilam, Iran

Abstract:

The GSP protein belongs to a big family of seed storage protein in cereal seeds. Grain hardness has a great influence on the process properties of wheat to bread or biscuits. In this study we Re-sequenced the GSP gene in eight wild wheat accessions with A genome related to two spices (Triticum dicoccoides and T. urartu) that have been collected in the different region of Iran. The results of PCR showed that GSP gene is present in all of the examined samples and produced 570 bp, although the length of the PCR product on Agarose gel shows high polymorphism. The results of the GSP gene sequencing in samples indicated that Triticum diccocoides(73.8) and T. urarto (37.8) show highest and lowest Similarity with bead wheat at NCBI respectively. Indeed, the cluster analysis shows that the samples are divided into two main groups, although they could not separate the samples by species that have been collected from different region of Iran. In general, bioinformatics analysis showed that significant differences in the size and length of the Gsp gene in compare to NCBI sequence. In order to get a deep understanding of this variation in the wild genetic resources of Iran and western Iran, which is a potential source for the desired quality germs, it should be carefully studied via genetically and biochemical characteristic.

Keywords:

Wild wheat, Gsp gene, Quality

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COI code: CIGS15_326

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Mansourian, Maryam; Arash Fazeli & Hamid Hosseinan Khoshro, 2018, Allelic variation of GSP gene in some wild wheat with A genome, The Third International and 15th National Genetics Congress, تهران, انجمن علمي ژنتيك ايران, https://www.civilica.com/Paper-CIGS15-CIGS15_326.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Mansourian, Maryam; Arash Fazeli & Hamid Hosseinan Khoshro, 2018)
Second and more: (Mansourian; Fazeli & Hosseinan Khoshro, 2018)
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Type: state university
Paper No.: 3250
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