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The use of phosphinothricin resistance as selectable marker for Nicotiana tabacum engineering

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Year: 2018
COI code: CIGS15_347
Paper Language: English

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Authors The use of phosphinothricin resistance as selectable marker for Nicotiana tabacum engineering

  Maryam Jamshidnia - Sari Agricultural Sciences and Natural Resources University, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Sari, Iran.Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, German Research Center for Environmental H
  Sayed Kamal Kazemitabar - Sari Agricultural Sciences and Natural Resources University, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Sari, Iran
Christian Lindermayr - Institute of Biochemical Plant Pathology, Helmholtz Zentrum München, German Research Center for Environmental Health, Munich, Germany
  Hamid Najafi Zarini - Sari Agricultural Sciences and Natural Resources University, Department of Plant Breeding and Biotechnology, Faculty of Agriculture, Sari, Iran

Abstract:

Phosphinothricin (PPT) is the active component of a family of environmentally safe, nonselective herbicides. The bar gene conferring tolerance to the herbicide phosphinothricin (PPT) encodes phosphinothricin acetyltransferase (PAT). Phosphinothricin Acetyl Transferase that detoxifies Phosphinothricin (PPT), the active ingredient of herbicides such as BASTA. We report here a transformation procedure with the bar gene as a selectable marker established via Agrobacterium- mediated transformation. It was introduced into the tobacco genome at a targeted site by homologous recombination. Transgenic plants of Nicotiana tabacum were regenerated by leaf disc transformation using a bar gene as a selectable marker. Transgenic plantlets were selected in medium supplemented with PPT (up to 0.1 mM). The transgenic N. tabacum plants resistant to PPT were positive upon PCR by bar gene specific primers. The transformed plants transferred to a greenhouse proved to be resistant to 1% PPT. The technology demonstrated here could perhaps be usefully transferred to other crop species.

Keywords:

Bar; Nicotiana tabacum, Transformation, Phosphinothricin.

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COI code: CIGS15_347

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Jamshidnia, Maryam; Sayed Kamal Kazemitabar; Christian Lindermayr & Hamid Najafi Zarini, 2018, The use of phosphinothricin resistance as selectable marker for Nicotiana tabacum engineering, The Third International and 15th National Genetics Congress, تهران, انجمن علمي ژنتيك ايران, https://www.civilica.com/Paper-CIGS15-CIGS15_347.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Jamshidnia, Maryam; Sayed Kamal Kazemitabar; Christian Lindermayr & Hamid Najafi Zarini, 2018)
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Type: state university
Paper No.: 5712
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