MicroRNA-143 inhibits cell migration and invasion lung cancer lines

Background: MicroRNAs (miRNAs) are an extensive family of small (18–24 nucleotide), endogenous, single-stranded non-coding RNAs. which target the 3 -untranslated region (3 -UTR) of specific mRNAs to promote their degradation or repression of translation. miRNAs regulate important cellular processes such as proliferation, apoptosis, mobility, cell cycle progression, and differentiation, and their altered expression is associated with various cancers. Among those miRNAs, miR-143 shows tumor-suppressive activity in some human cancers. Lung cancer is the leading cause of cancer deaths worldwide and metastasis is the major cause of death in lung cancer patient. Methods: Human lung cancer cells (A549) were cultured at 37 C in 5% CO2 with RPMI 1640 media supplemented with 10% fetal bovine serum (FBS). Transient transfection of miRNA precursors or inhibitors was carried out using Lipofectamine 2000 according to the manufacturer’s protocol. Migration of human lung cancer cells in culture was determined by the scratch assay. For this, cells were seeded into a six well tissue culture dish. Cells in monolayers were scratched in a single straight line using a pipette tip. The migratory distance was measured under a microscope equipped with a camera.Results: Intrinsic miR-143 expression was significantly decreased in lung cancer cells compared to non cancerous epithelial cells. Restoration of miR-143 led to inhibit migration and invasion of human lung cancer cells. These data suggest that miR-143 suppress pathways relevant to tumorigenicity and cancer progression.Conclusion: The existing experimental evidence suggests that it is worth testing Mirna-143 as a cancer therapeutic agent since the results of this study demonstrate that Mirna-143 has the ability to inhibit migration and invasion of human lung cancer cells.

Keywords:

Lung cancer , MicroRNAs , Mirna-143 , Scratch assay