Evaluating expression level of circulating miRNA-103 in Polycystic Ovary Syndrome as a non-invasive biomarker

Background: Polycystic ovary syndrome (PCOS) has a largely unknown etiology and a common heterogeneous endocrine disorder in women of reproductive age, is characterized by polycystic ovaries, hyperandrogenism, insulin resistance and chronic anovulation. MicroRNAs (miRNAs) are small, noncoding RNA sequences that negatively regulate gene expression at the post-transcriptional level. miRNA-103 have been associated with metabolic disorders such as obesity and diabetes, which are also associated with polycystic ovary syndrome (PCOS).Objective: This study aims to describe different expression of circulating miR-103 in plasma of PCOS women.Material and Methods: This is a Case–control study, include 10 women with PCOS and 10 healthy women. Plasma total RNA was isolated and after poly adenylation convert to total cDNA. Then expression level of microRNA-103 was analyzed by Q-PCR and Snord miRNA used as reference gene. Result and Conclusion: Our results indicates the expression level of miRNA-103 significantly decreased in PCOs group comparing to healthy women (p<0.05). According to our finding, further studies are recommended in the larger statistical population to confirm microRNA-103 as a non-invasive diagnosis biomarker.

Keywords:

polycystic ovary syndrome (PCOS) , microRNA-103 , biomarker , Q-PCR