Effect of Cold and Methyl Jasmonat on Expression mir319 and its Target Gene TCP in two Wheat Cultivars

Cold is the main abiotic stress that limits cultivation and reduce yield of wheat (Triticum aestivum L.) in the world [1]. miR319 is one of the conserved microRNA families that controls transcription factors of TCP family involved in hormone biosynthesis and signaling, different developmental pathways, such as senescence and leaf development, cell proliferation and differentiation in plants [2]. miR319 expression is controlled by abiotic stress, however experimental evidence is insufficient and the mechanism remains unknown. This study investigate the expression of miR319 and it s target gene (TCP) in two wheat cultivars, Norstar and Baz, exposed to cold stress (4 °C) for 0, 2, and 14 days and methyl jasmonate (0 and 120 µM). Our results demonstrate that miR319 expression is down-regulated in Norstar plantlets 4 °C for 2days, but up-regulated in the spring wheat cultivar baz. miR319 was also controlled by treatment with methyl jasmonate. miR319 expression decreases in winter wheat cultivar, Norstar, under the influence of methyl jasmonate. Initially, TCP expression was reduced by cold treatment (2 days), but with longer periods of time of cold treatment (14 days), the expression increases. In general, the expression of miR319 and TCP was regulated during cold stress and methyl jasmonate treatment.

Keywords:

cold , miRNA , methyl jasmonate , TCP