Bioinformatic Evaluation of the miR-1245 Effect on ERBB2 Signaling Pathway

MiRNAs, a small noncoding RNA of 21–22 nucleotides long, have recently been linked to cancer development. miRNAs have diverse functions, which include the regulation of cellular differentiation, proliferation, and apoptosis. The available evidence has shown that miRNAs widely participate in the development or progression of many types of cancers, including breast cancer. Breast cancer is the most common cancer in women. Among the genes that may be potentially affected by miRNA, ERBB2 is perhaps the best known. This gene is expressed at a low level in normal human tissues. However, when it is overexpressed, it produces the malignant phenotype and leads to cell proliferation. Approximately 25% of human breast cancers overexpress the HER2 proto-oncogene, and these breast cancers have a more aggressive tumor phenotype and produce a poor prognosis in patients with this disease.Methods: Using different algorithms in TargetScan, DIANA and miRWalk databases, targets of miR-1245 were identified. Then, a score table was prepared from the candidate genes, based on the affinity of the seed region of miR-1245 and the number of targets in the 3 -UTR region of targets.Results: The results of bioinformaical analysis showed that the POU1F2, KLF6, ERBB2,SOX9, SP1, and EFNB1 molecules are the most potential targets that might be affected by miR-1254.Conclusion: It seems that miR-4430 might be a potential regulator of ERBB2 expression in breast cancer. Therefore, this protein can be considered as a suitable new candidate for experimental evaluation.

Keywords:

Bioinformatics , microRNA , Breast cancer , miRNA