The bioinformatics of the interaction of the allele APC in rs 3733961 with microRNA 155 in gastric cancer

Gastric cancer is one of the most common cancer around the world[1]. Adenomatous polyposis coli or APC, has been known as a tumor suppressor gene. inactivation of APC causes the development of some gastric cancers, and mutations of the APC gene, occur during the early stages of gastric adenoma development [2] .The APC gene inhibits the members of Wnt signalling pathway that promotes β-catenin expression as a stimulator of cell division within the intestinal[2].MicroRNAs contribute to gastric carcinogenesis by altering the expression of oncogenes and tumor suppressors, affecting cell proliferation, apoptosis, motility, and invasion [3].material and method miRNASNP2 database was used to identify the miRNAs with the ability to bind to the 3’UTR of APC transcripts. Then, miRTarbase and David were used to investigate the function and related pathways of obtained miRNA and relationship with APC. We, by kegg database, observed pathways in which miR-155 and APC are involved in cancers. Altogether, these stages helped us to find relationship between pathways of apc and mir-155.Results In silico investigation of SNPs in the 3’UTR of APC gene showed that rs3733961 could alter the binding properties of miR-155. due to rs3733961, the binding activity of miR-155(as an tumor suppressor) undergoes loss; consequently, this SNP could act as a poor-prognostic factor. On the other hand, the binding affinity of miR-155 alleviates as a result of rs-3733961; therefore, rs-3733961 could also act as good-prognostic factor.Conclusion Bioinformatically, rs3733961 could have association with gastric cancer especially with prognosis of patients.