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Comparative Expression of microRNAs in Young-Cardiomyocyte and hESC- Cardiomyocytes by bioinformatics methods

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Year: 2018
COI code: CIGS15_564
Paper Language: English

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Authors Comparative Expression of microRNAs in Young-Cardiomyocyte and hESC- Cardiomyocytes by bioinformatics methods

  Akram Gholipour - Department of Biology, Science and Research branch, Islamic Azad University, Tehran, Iran
  Elham Taheri - Department of Molecular Genetics, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
  Ali Sharifi Zarchi - Department of Computer Engineering, Sharif University of Technology, Tehran, Iran
  Shiva irani - Department of Biology, Science and Research branch, Islamic Azad University, Tehran, Iran
  farshad Shakerian - Cardiogenetic Research Center, Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran
  ali Zahedmehr - Cardiogenetic Research Center, Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran

Abstract:

Introduction: Cardiac development is precisely controlled by complex regulatory networks. Recent reports demonstrated that microRNAs (miRNAs) act as micromanagers of gene expression at all stages of cardiac development. In this study, using different bioinformatics tools, we aimed to determine mRNAs and miRNAs expression profiles in human embryonic stem cell-derived cardiomyocytes (hESC-CMs). Methods: mRNAs and miRNAs were compared between 1-year matured hESC-CMs, and differentiated cardiomyocytes at day 20 (young-CM) samples of GSE62913. Then, differentially expressed mRNAs and miRNAs with padj<0.05 and log2FoldChange≠1 were chosen to perform pathway analysis. Pathway enrichment analysis and Regulatory Network were accomplished by Enrichr database and CytoscapeV3.6.0, respectively. In addition, miRWalk database was utilized to analyze the target genes of the highly expressed miRNAs.Results: Our data exhibited 2138 mRNAs and 172 miRNAs with different expression pattern. Pathway analysis and regulatory Network depicted that differentially expressed genes are involved in: complement and coagulation, cardiac progenitor differentiation, dilated cardiomyopathy, hypertrophic, and cardiac muscle contraction pathway. In the following, among differentially expressed miRNAs, hsa-miR-98-5p and hsa-miR-122-5p had the highest level of altered expression and were chosen for experimental validation. The upregulated hsa-miR-98-5p and downregulated hsa-miR-122-5p target the 3´-UTR of MTUS1 and FUNDC2 genes. The latter genes show high level of expression in artery and heart.Conclusion: All in all, cardiac development and cardiomyocyte maturity are very complicated. Determining the Cardiomyocyte-related miRNAs and their targets would shed more light on molecular processes of cardiac development.

Keywords:

Differentially expressed miRNAs; Young-Cardiomyocyte; hESC-CMs

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COI code: CIGS15_564

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Gholipour, Akram; Elham Taheri; Ali Sharifi Zarchi; Shiva irani; farshad Shakerian & ali Zahedmehr, 2018, Comparative Expression of microRNAs in Young-Cardiomyocyte and hESC- Cardiomyocytes by bioinformatics methods, The Third International and 15th National Genetics Congress, تهران, انجمن علمي ژنتيك ايران, https://www.civilica.com/Paper-CIGS15-CIGS15_564.htmlInside the text, wherever referred to or an achievement of this article is mentioned, after mentioning the article, inside the parental, the following specifications are written.
First Time: (Gholipour, Akram; Elham Taheri; Ali Sharifi Zarchi; Shiva irani; farshad Shakerian & ali Zahedmehr, 2018)
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