Verification human gender identity with TDF and DQα genes and polymerase chain reaction technique

Nowadays sex determination is one of the most important and practical methods in Molecular Biology filed which is the main improvement in Health and welfare, Agriculture industry, Forensic Science and Archaeology. Achieving suitable methods to economize and saving time by regarding user safety is the purpose of this study. In this study, PCR technique, human TDF and DQα genes sequence are used. In blood samples which are provided by Tabriz Hakim Laboratory, 50 volunteers selected without considering their sex. From each volunteer 5cc blood was taken and DNA extracted. DNA quantity and quality checked with both Spectrophotometry and Electrophoresis on the agarose gel. PCR method used for amplification,DNA sequences by special primers for TDF and DQα genes which have a 139bp and 239bp size in order. In female samples we find just one sharp band (239bp) that related to X chromosome (DQα gene), in male samples we find two sharp bands (239 and 139pb) that related to Y (TDF gene) and X (DQα) chromosomes. At the end, the results were analyzed by Chi-Square test. The test shows complete conformity between expected and observed results. This technique is able to determine the sex in human samples with high accuracy and this method is economical so it can be a candidate for commercial method.

Keywords:

Sex determination , PCR , TDF , DQα